微波消解-原子荧光光谱法测定饲料中的砷、汞、硒的研究

Determination of As,Se and Hg in feed by microwave digestion-atomic fluorescence spectroscopy

采用微波消解-原子荧光光谱法对饲料中砷、汞、硒的测定条件(仪器条件的选择、不同酸体系的选择、酸浓度、硼氢化钾浓度等)进行了研究。结果表明:采用HNO3-H2O2对饲料进行微波消解;用体积分数为5%的HCl做载流酸,质量浓度20g/L的硼氢化钾做还原剂测砷、硒,质量浓度5g/L的硼氢化钾测汞;在砷、汞、硒标准溶液质量浓度均为0～10μg/L范围内,砷、汞、硒校准曲线的相关系数均大于0.999 2,且加标回收率均在90%～105%。

The determination conditions of As, Se and Hg in feed by microwave digestion-atomic fluorescence spectroscopy were researched, including the choice of instrument conditions, the choice of different acid system, acid concentration, the concentration of potassium borohydride, etc. The results indicated that：The microwave digestion with HNO2 - H2O2 had many advantages such as fast and efficient, less reagent, low blank, less sample volatilization, etc; With 5 % （v/v） HC1 as current-car rying acid, 20 g/L potassium borohydride as the reducing agent for As and Se, and 5 g/L potassium borohydride as the reducing agent for Hg, the calibration curve correlation coefficients of As, Se and Hg were all greater than 0. 999 2 and the recovery fraction was 90%-105 %