摘要
采用构建16S rDNA克隆文库方法对活性污泥系统的细菌种群多样性进行研究。随机测定了71个克隆子序列(1 500bp),BLAST比对结果表明,活性污泥中微生物群落具有高度多样性,可分为6个主要类群,其中,拟杆菌(Bacteroides)类群和γ变形菌(γ-Proteobacteria)类群在文库中所占比例最大,分别为38.03%和32.39%;其次是β-Proteobacteria,占19.72%;Firmicutes,Candidate division TM7和α-Pro-teobacteria类群所占比例相对较小,分别为4.23%,4.23%和1.04%。序列分析结果表明,活性污泥中具有可强化生物脱氮除磷效果的食酸菌(Acidovorax),包括假单胞菌(Pseudomonas),红环菌(Rhodocyclus)等细菌。
The bacterial diversity of activated sludge was studied by the construction of a 16S rDNAclone library.71 clones were randomly selected from the library.The corresponding partial 16S rDNAgene（ca.1 500 bp） was sequenced and blasted.The results indicated that the bacterial co mMunity in the activated sludge was highly diverse,and could be divided into six major groups.The largest fractions were Bacteroides and γ-Proteobacteria,which accounted for 38.03% and 32.39% of the total clones,respectively.The portions of β-Proteobacteria was 19.72%,respectively.The relatively lower portions were 4.23% Firmicutes,4.23% Candidate division TM7 and 1.04% α-Proteobacteria.The result of sequence analysis also shows that there are Acidovorax,Pseudomonas and Rhodocyclus,which strengthen biological denitrification and phosphorus effect in the activated sludge
