豚鼠膀胱二聚体cajal样细胞形态及其功能的结构基础

The special types and structural of function of dimer cajal-like cell in the bladder of guinea pig

目的观察豚鼠膀胱组织中二聚体cajal样细胞形态学特点,探讨二聚体cajal样细胞在膀胱组织中发挥功能的结构基础。方法 15只豚鼠膀胱组织经手术取材后制成冷冻切片和电镜切片,分别经免疫荧光染色后行激光共聚焦显微镜和电镜观察。采用酶消化法原代培养,电镜观察培养24h的ICCs超微结构。结果激光共聚焦显微镜下见豚鼠膀胱组织中cajal样细胞有双极和二聚体两种类型,cajal样细胞二聚体紧密连结形成相通荧光缺陷通道,两类cajal样细胞存在网络联系。电镜下cajal样细胞与平滑肌细胞和神经纤维之间存在广泛的缝隙连结。培养后cajal样细胞间可见桥样结构。结论豚鼠膀胱组织中存在双极和二聚体两种不同类型的cajal样细胞,二聚体cajal样细胞形成的相通荧光通道样结构可能是其发挥起博功能的结构基础,在膀胱中主要起起博功能,二聚体cajal样细胞与双极cajal样细胞构成的广泛网络联系,以及与平滑肌细胞和神经纤维之间形成的大量缝隙连结,是该类cajal样细胞具备信息传递的结构基础,在膀胱组织中主要起信息传递功能。

Objective To observe the morphological feature of dimer cajal-like cells in the bladder of the guinea pig,in order to describe the structural basis of dimer cajal-like cells in bladder trissues to play function.Methods Fifteen guinea pig bladder tissues were made frozen sections and electron microscopic section after the surgery,then frozen sections were done immunofluorescent staining and observed by laser scanning confocal microscope.Primary culture ICCs bladder cells（24 hours） with collagenase digestion in vitro.To observe their ultrastructural by transmission electron microscope.Results Cajal-like cells had two styles of bipolar and dimer,close connection of dimer cajal-like cells forms the channel of fluorescence defect.There was an extensive network between bipolar cajal-like cells and dimmer cajal-like cells.Bipolar cajal-like cells contact to smooth muscle cells and nerves by abundant gap connection.Cultured cajal-like cell had bridge structures between them.Conclusion The bladder of guinea pig exists two styles of dimer and bipolar cajal-like cells.The channel of fluorescence defect of dimer cajal-like cells may be the structural basis that they play pacemaking function in bladder.The extensive network and abundant gap connection that formed by dimer and bipolar cajal-like cells are the structural basis of information transmission,play main function for the transmission of information in the bladder of guinea pig.