高危型HPV检测联合肉眼醋酸试验在宫颈癌前病变筛查中的应用

The application of HPV-DNA detection combined with VIA test in screening of precancerous lesion of uterine cervix

目的:研究在宫颈癌前病变筛查中采用高危型HPV检测联合肉眼醋酸试验的应用效果。方法:选取2007年8月～2010年2月于我院治疗的可疑宫颈癌前病变患者532例,应用5%醋酸和Lugol's液涂抹于宫颈表面,筛选出有异常着色的患者320例,再采用实时荧光定量PCR技术检测高危型HPV病毒感染情况。结果:320例肉眼醋酸试验异常着色患者进行高危型HPV病毒感染检测发现,HPV阳性感染190例,阳性率为59.38%;212例肉眼醋酸试验正常着色患者进行高危型HPV病毒感染检测发现,HPV阳性感染18例,阳性率为8.49%。结论:肉眼醋酸试验联合高危型HPV检测可及时、有效地发现宫颈癌前病变,可作为宫颈癌前病变筛查方案应用于临床。

Objective： To study the efficiency of HPV-DNA detection combined with visual inspection with acetic acid（VIA） test in screening of precancerous lesions of uterine cervix.Methods： 532 suspected precancerous lesion of uterine cervix patients in our hospital from August 2007 to February 2010 were selected.5% acetic acid and Lugol＇s liquid were painted on the surface of cervix of all patients,and 320 cases of patients were screened by the abnormal color.Then the real-time fluorescence quantitative polymerase chain reaction technology was used to test high risk HPV infection in all patients.Results： High risk HPV infection test of 320 patients who were showed abnormal color in VIA test was showed that 190 cases got HPV positive infection,the positive rate was 59.38%.High risk HPV infection test of 212 patients who were showed normal color in VIA test was showed that 18 cases got HPV positive infection,the positive rate was 8.49%.Conclusion： VIA test combined with HPV-DNA checking can timely and effectively discover precancerous lesion,which can be used in clinic as precancerous lesion screening plan.