扁平苔藓中早期生长反应因子-1和肿瘤坏死因子-α的表达及意义

Expression and significance of early growth response-1 and tumor necrosis factor-α in lichen planus

目的:探讨早期生长反应因子-1(Egr-1)和肿瘤坏死因子-α(TNF-α)在扁平苔藓发病中的作用。方法:采用TUNEL原位凋亡检测法检测30例扁平苔藓皮损及30例正常皮肤组织中细胞凋亡情况,并用免疫组织化学法检测该30例扁平苔藓和30例正常皮肤组织中Egr-1和TNF-α的表达情况。结果:扁平苔藓皮损处角质形成细胞和真皮浅层浸润的淋巴细胞中细胞凋亡水平明显高于对照组;在相同部位,Egr-1和TNF-α的表达水平高于正常皮肤组织,差异有统计学意义(P<0.05),且二者在扁平苔藓皮损处的表达呈正相关(r=0.896 7,P<0.05)。结论 :Egr-1和TNF-α的高表达可能参与了扁平苔藓的发病。

Objective： To investigate the effect of Egr-1（early growth response-1） and TNF-α（tumor necrosis factor-α） on the pathogenesis of lichen planus.Methods： TUNEL was used to detect the apoptotic cells,and the immunchistochemical technique was used to test the expression of Egr-1 and TNF-α in 30 LP lesions and 30 normal skins.Results： Compared with the control group,the number of apoptotic cells was significantly increased in the ketatinocytes and infiltrating lymphocytes of lichen planus.In the same site,the expression of Egr-1 and TNF-α in lichen planus was significantly stronger than that in the normal skins（P0.05）,and there were positive correlations between the expression of Egr-1 and TNF-α in LP lesions（r=0.896 7,P0.05）.Conclusion： Egr-1 and TNF-α may participate in the pathogenesis of lichen planus.