摘要
非人灵长类动物是十分重要的生物医学资源。由于与人类在生理生化、免疫、遗传等方面近似,猕猴是重要的非人灵长类实验动物之一。然而,猕猴作为自然宿主,易感染D型逆转录病毒(simian type D retrovirus,SRV)和T淋巴细胞白血病病毒(simian T lymphotropic virus,STLV)这两种逆转录病毒,并可能会影响AIDS猕猴动物模型等的研究结果。猴B病毒(ceropithecine herpesvirus1,BV)对猕猴及动物从业人员均有危害。云南省拥有较大规模的中国猕猴繁殖种群。基于以上原因,建立SPF级别的中国猕猴种群十分必要。该文应用PCR技术筛查了人工饲养种群中411只中国猕猴的SRV、STLV和BV感染流行情况。结果表明:SRV、STLV和BV的阳性感染率分别为19.71%(81/411)、13.38%(55/411)和23.11%(95/411)。同时比较分析了不同性别及年龄组中国猕猴的病毒感染情况。该研究将有助于建立SPF级别的中国猕猴繁殖种群。
Nonhuman primates are critical resources for biomedical research.Rhesus macaque is a popularly used laboratory nonhuman primate that share many characteristics with humans.However,rhesus macaques are the natural host of two exogenous retroviruses,SRV(simian type D retrovirus) and STLV(simian T lymphotropic virus).SRV and STLV may introduce potentially significant confounding factors into the study of AIDS model.Moreover,B virus(ceropithecine herpesvirus 1) is likely to harm not only rhesus macaque but also humans in experiments involving rhesus macaque.Yunnan province has large-scale breeding colonies of Chinese rhesus macaque.Therefore there is an urgent need for SPF Chinese rhesus macaque colonies.Here we investigated SRV,STLV and BV infections in 411 Chinese rhesus macaque by PCR technique.The results showed that the prevalence of SRV,STLV and BV among Chinese rhesus macaque breeding colony was 19.71%(81/411),13.38%(55/411) and 23.11%(95/411),respectively.Comparison of viruses infection in different age-groups and male/female of Chinese rhesus macaque was also analyzed.This study will contribute to establishment of SPF Chinese rhesus macaque breeding colony.
基金
国家科技重大专项课题(2009ZX09501-029
2012ZX10001-007)
中国科学院重要方向课题(KSCX1-YW-10
KSCX2-EW-R-13)
"西部之光"人才计划项目