FTY720对Hep-2细胞增殖抑制及诱导程序性死亡作用

Inhibition of proliferation and induction of programmed death of Hep-2 cells by FTY720

目的探讨FTY720对体外培养的Hep-2细胞增殖抑制及诱导程序性死亡作用。方法用不同浓度的FTY720(800、1 600、3 200 ng/ml)处理Hep-2细胞,48 h后,采用MTT法检测细胞的增殖活力;瑞士-姆萨染色观察细胞形态的变化;流式细胞术检测细胞周期及凋亡情况。结果 FTY720对Hep-2细胞增殖的抑制率呈剂量依赖性,当FTY720浓度为3 200 ng/ml时,Hep-2细胞的增殖受到明显抑制,抑制率为(60.900±0.071)%(P<0.05);经FTY720处理的细胞出现程序性死亡形态;FTY720可将Hep-2细胞阻滞于G2期,1 600 ng/ml FTY720组细胞的凋亡率明显升高(P<0.05)。结论一定浓度的FTY720能明显抑制体外培养的Hep-2细胞增殖,调节细胞周期,并诱导其发生程序性死亡。

Objective To investigate the inhibition of proliferation and induction of programmed death of Hep-2 cells cultured in vitro by FTY720. Methods Hep-2 cells were treated with FTY720 at various concentrations （800, 1 600 and 3 200 ng/mt） for 48 h, .then observed for proliferation activity by MTY assay, for morphology by Switzedand-Giemsa staining, and for cell cycle and apoptosis by flow cytometry. Results FTY720 showed dose-dependent inhibitory effect on proliferation of Hep-2 cells. The inhibiting rate of cell proliferation by FTY720 at a concentration of 3 200 ng/ml was （60. 900±0. 071）% （P 〈 0. 05）. Programmed death was observed in the cells treated with FFY720 which arrested the cells at G2 phase. The apoptosis rate of cells treated with 1 600 ng/ml FTY720 increased significantly （P 〈 0. 05 ）. Conclusion The FTY720 at a certain concentration inhibited the proliferation, regutated the cell cycle and induced the programmed death of Heo-2 cells cultured in vitro.