全细胞差减筛选法在噬菌体展示文库筛选中的应用

Application of whole-cell subtractive panning in phage display library screening

全细胞差减筛选法是近年来在细胞筛选的基础上发展起来的一项筛选技术,其利用成对的细胞,即两种不同状态的细胞对噬菌体展示文库进行差减筛选,主要用于筛选新的抗原表位、受体、配体、新型疫苗、肿瘤细胞的靶向活性肽、靶向基因载体及受体或酶的激动剂或抑制剂等。本文就其筛选的原理、优缺点、优化及其在噬菌体展示文库筛选中的应用作一综述。

Whole-cell subtractive panning is a screening technique developed based on whole-cell screening in recent years, which performs subtractive panning on phage display library by using couple cells, i.e. two states of cells. It is mainly used for screening of novel antigenic epitopes, receptors, ligands, novel vaccines, the targeted active peptides of tumor cells, targeted gene vectors, and stimulants or antagonists of receptors or enzyme etc. The principle, advantages, disadvantages and optimization of the technique as well as its application in phage display library screening are reviewed in this paper.