印记基因Dlk1在小鼠胚胎发育中的表达分析

Expression of Imprinting Gene Dlk1 During Mouse Embryogenesis

目的:研究印记基因Dlk1在小鼠胚胎发育过程中的动态表达模式,以揭示Dlk1与胚胎发育的关系。方法:通过半定量PCR和定量PCR分析Dlk1在小鼠胚胎发育E8.5～E19.5的基因表达模式,并选取Dlk1表达量最高的时期进行胚胎切片原位杂交和组织定量PCR分析。结果:在小鼠胚胎发育E8.5～E15.5时,Dlk1的表达逐渐升高,在E15.5时表达量达到最高;E15.5～E19.5时,Dlk1表达有所下降,但仍然维持较高水平。E15.5切片原位杂交显示,垂体、肺脏、软骨、舌和背侧肌肉组织中Dlk1表达较高,组织定量PCR实验进一步证实了原文杂交的结果。结论:Dlk1在小鼠胚胎发育中后期持续表达,并呈现一定的组织特异性,对胚胎发育可能起重要的调节作用。

Objective： To illustrate the dynamic expression of imprinting gene Dlk1 during mouse embryogenesis on mRNA level.Methods： The reverse transcription PCR（RT-PCR） and quantitative real time PCR（qRT-PCR） were performed to analyze the expression of Dlk1 during mouse embryogenesis.Further characterization was carried out by in situ hybridization（ISH） and qRT-PCR on the stage when Dlk1 expression reached its peak.Results： The expression of Dlk1 was gradually up regulated during E8.5 through E19.5,then it was slightly down regulated,suggesting a peak at E15.5.ISH data indicated that Dlk1 was strongly expressed in the pituitary,tongue,dorsal muscle and cartilage system.This result was further certificated by qRT-PCR in E15.5 tissues.Conclusion： The imprinting gene Dlk1 was persistently expressed during mid-late stage of mouse embryogenesis,suggesting a possible role in mouse embryo development.