SDS-PAGE分析肝癌细胞和正常肝细胞膜蛋白

SDS-PAGE Analysis of Membrane Protein of Hepatoma Carcinoma Cells and Normal Liver Cells

目的:采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法获得肝癌细胞株HepG2和人正常肝细胞株L-02蛋白质表达谱,期待找到与肝癌细胞相关的差异蛋白。方法:体外培养肝癌细胞株HepG2和正常肝细胞株L-02,分离提取细胞膜蛋白,用SDS-PAGE分析细胞膜蛋白。结果:2种细胞膜蛋白的疏水相和亲水相蛋白区带位置基本相同,但肝癌细胞株HepG2膜蛋白在相对分子质量66×103处有较清晰的异常蛋白区带。结论:肝癌细胞HepG2除了具有与正常肝细胞相同的组成物质外,还有自己独特的蛋白质表达谱,这一实验可为癌症的研究提供一定的参考。

Objective： Screening the protein express spectrum of hepatoma carcinoma cells（HCC） HepG2 and normal liver cells L-02 by SDS-PAGE,we expect to find out the HCC related protein.Methods： The membrane proteins of HepG2 and L-02 cells cultured in vitro were abstracted,and were analyzed by SDS-PAGE.Results： The positions of protein bands both in hydrophobic and hydrophilic membrane protein were basically the same,but the protein bands of HepG2 membrane protein were distinctly abnormal at 66 kD.Conclution： Besides having the same substance with normal liver cells,HCC HepG2 has its own distinctive protein express spectrum.This experimentation may offer some references for research on cancer.