蒽贝素(Embelin)通过阻断NF-κB信号通路抑制肝癌细胞Bel-7404生长的研究

Suppression of Hepatoma Carcinoma Cell Bel-7404 by Small Molecule XIAP Inhibitor Embelin Through Blocking NF-κB Signaling Pathway

蒽贝素(Embelin)是一种X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)的小分子抑制剂,可以抑制XIAP的生成和活性,从而解除XIAP的抗凋亡作用,使凋亡顺利进行.研究了Embelin抑制肝癌细胞Bel-7404增殖的机制.采用不同浓度梯度,通过荧光显微镜、Hochest33342染色、MTT检测、AnnexinⅤ/PI流式细胞术和Western blot分别检测Embelin对Bel-7404细胞的形态学变化、凋亡小体形成、细胞存活率、细胞凋亡水平、凋亡信号转导相关蛋白表达的影响.结果显示,Embelin能明显抑制Bel-7404细胞增殖,并通过激活caspase通路以及阻断NF-κB信号通路诱导Bel-7404细胞凋亡,为进一步利用Embelin进行肝癌治疗的研究打下一定的基础.

Embelin,a small molecule XIAP（X-linked inhibitor of apoptosis protein） inhibitor,can inhibit the expression and activation of XIAP,thereby removing the antiapoptotic ability of XIAP and resulting in apoptosis process smoothly.This study is attempt to explore the mechanism of the inhibition to hepatocarcinoma cell Bel-7404 growth by Embelin.After treated with different-concentration of Embelin,the morphological change,formation of apoptotic bodies,cells viability,cell apoptosis and the apoptosis-related protein expression in Bel-7404 cells were assessed by fluorescence microscope,Hochest33342 staining,MTT assay,Annexin Ⅴ/PI flow cytometry and Western blotting et al.The results indicated that Embelin remarkably inhibited human hepatocarcinoma cell Bel-7404 growth.Further experiments found that the mechanism of tumor cell growth inhibition by Embelin was due to the cell apoptosis with the activation of caspase apoptotic pathway and blockage of NF-κB signal pathway.The studies laid a base on the further treatment of human hepatoma carcinoma by using Embelin.