补肾舒脊颗粒对强直性脊柱炎患者关节液成纤维细胞分泌白细胞介素6的影响

Effects of Bushen Shuji Granule on IL-6 in the Culture Fluid Sample of Fibroblast Cells from the Synovial Liquid of the Ankylosing Spondylitis Patients

目的观察补肾舒脊颗粒对强直性脊柱炎(AS)患者关节液成纤维细胞分泌白细胞介素6(inter-leukin6,IL-6)的影响。方法运用血清药理学方法,选择不同浓度的含药血清与AS患者膝关节液体外培养成纤维细胞共同孵育,运用ELISA方法检测各组细胞分泌IL-6水平,并运用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)方法从mRNA水平进一步分析IL-6的表达情况。并与空白血清、柳氮磺胺吡啶(Sulfasalazine,SSZ)血清组作对照。结果两组成纤维细胞培养上清中IL-6的ELISA检测结果显示:AS组成纤维细胞产生的IL-6较空白血清组显著性升高。给予补肾舒脊颗粒能明显抑制AS成纤维细胞分泌IL-6水平,与空白血清组比较,差异有统计学意义,并且补肾舒脊颗粒对成纤维细胞增殖的抑制作用有明显的量效关系。AS组IL-6的mRNA水平较空白血清组升高,半定量分析提示两组差异有统计学意义。结论补肾舒脊颗粒可通过抑制AS成纤维细胞分泌IL-6的水平,发挥其临床抗炎、抗纤维化的作用。

Objective To explore the effects of Bushen Shuji Granule（BSG） on inhibiting the interleukin 6（IL-6） level in the synovial fluid sample of fibroblast cells from the ankylosing spondylitis（AS） patients.Methods Using serum pharmacologic method,the IL-6 level in the culture fluid sample of fibroblast cells was observed by ELISA method with different concentrations of medicated serum containing BSG.The IL-6 level at the mRNA level was detected using reverse transcriptase-polymerase chain reaction（RT-PCR）.The vehicle serum and sulfasalazine（SSZ） serum were taken as controls.Results Results of ELISA showed the IL-6 level in the AS group was more than that in the vehicle serum group with obvious statistical difference.BSG could obviously inhibited the IL-6 level,showing statistical difference when compared with the vehicle serum group.Besides,obvious dose-dependent correlation existed between BSG and its inhibition on fibroblast proliferation.And the IL-6 level at the mRNA level in the AS group was higher than that in the vehicle serum group,showing statistical difference by semi-quantitative analysis.Conclusion BSG could play its clinical role of anti-inflammation and anti-fibrosis through inhibiting the IL-6 level in the culture fluid sample of fibroblast cells.