苔藓植物matK基因PCR反应体系的建立和优化

Establishment and Optimization of matK gene-PCR Amplification System for Bryophytes

[目的]建立和优化适合于苔藓植物matK基因的PCR反应体系。[方法]以鳞叶藓为材料,利用改良CTAB法提取了基因组DNA,利用单因素试验分析了DNA模板、引物和2×Taq MasterMix的浓度对苔藓植物matK基因PCR反应的影响,对适合苔藓植物的matK基因PCR反应条件进行了优化。[结果]适合苔藓植物的matK基因PCR反应体系为10.0μl,其中含DNA模板0.5μl,正反引物各0.2μl,2×Taq MasterMix 5.6μl。[结论]为苔藓植物的分子系统学等研究奠定了基础。

[Objective] To establish and optimize the matK gene-PCR reaction system for Bryophytes.[Method]Using genomic DNA of Bryophytes extracted via an improved CTAB method,single factor analysis was performed to investigate the impacts of DNA template concentration,primer concentration,2×Taq MasterMix concentration on matK gene-PCR amplification and to optimize this system for Bryophytes.[Result]The matK gene-PCR amplification（10.0 μl） suitable for Bryophytes was determined to be composed of 0.5 μl DNA,0.2 μl primer and 5.6 μl 2×Taq MasterMix.[Conclusion] The study laid basis for analyzing the molecular systematics of Bryophytes.