GICA、PAT和ELISA法联合应用快速诊断肺炎支原体感染方法探讨

Rapid and accurate diagnosis of mycoplasma pneumoniae infection by combination of GICA,PAT and ELISA assays

目的探讨3种肺炎支原体(mycoplasma pneumoniae,MP)快速检测方法即明胶颗粒凝集法(PAT)、胶体金免疫层析法(GICA)和酶联免疫吸附法(ELISA)法的各自临床诊断价值和优势,为临床合理选择使用这几种方法提供实验依据。方法同时用3种方法对临床疑似病人血清进行同步检测,采用四格表法对检测结果进行统计分析。结果 GICA与ELISA对MP诊断的敏感性和特异性高度一致;但这2种方法与PAT诊断结果的一致性较差。结论相对于ELISA方法,GICA法由于其经济、有效,更适合MP的早期快速筛查;虽然GICA/ELISA法与PAT法诊断结果的一致性较差,但它们的联合使用有助于快速判断MP感染时期,可为该病的治疗方案提供实验依据。

To investigate respective advantages of the particle agglutination test（PAT）,gold Immunoc-hromatography assay（GICA） and enzyme linked immunosorbent assay（ELISA） for diagnosis of mycoplasma pneumoniae（MP） infection,we simultaneously used the three methods to detect the serum antibody and compared the coincidence between these methods by using the fourfold table method.Results showed that the coincidence between PAT and GICA assays was very low,in contrast,the coincidence between GICA and ELISA assays was markedly high.These results suggest that compared to ELISA,GICA assay is more suitable for rapid diagnosis of MP infection,due to its economy and effectiveness.Though there is low coincidence between GICA and PAT assays,their combination application would be very helpful for rapid identification of different phases of MP infection,and thus provide experimental basis for the design of treatment of this disease.