银屑病患者CDKN2A甲基化状态研究

CDKN2A gene methylation in patients with plaque psoriasis

目的探讨银屑病患者表皮CDKN2A基因甲基化状态及其在疾病发生机制中的作用。方法采用甲基化特异性PCR技术对48例斑块状银屑病患者表皮(银屑病组)和18例外观正常皮肤表皮(对照组)进行CDKN2A基因甲基化的分析。结果银屑病组CDKN2A基因甲基化状态(37.5%)明显高于对照组(11.1%),差异有统计学意义(P<0.05);斑块状银屑病患者表皮CDKN2A甲基化阳性者PASI积分与阴性者比较差异有统计学意义(P<0.01)。结论斑块状银屑病患者CDKN2A基因呈高甲基化状态,可能参与斑块状银屑病的发病机制。

Objective To study the state of CDKN2A gene DNA methylation in patients with plaque psoriasis and its significance in the pathogenesis of the disease.Methods The methylation status of CDKN2A gene was detected in 48 plaque psoriasis patients and 18 healthy controls with methylation-specific polymerase chain reaction.Results The rates of CDKN2A gene hypermethylation was higher in psoriatic patients than that in the controls,which showed a significant difference（P0.05）.There was a significant difference in PASI between the patients with positive CDKN2A gene DNA methylation and the patients with negative CDKN2A gene DNA methylation（P0.01）.Conclusion CDKN2A hyper-methyIation may be involved in the pathogenesis of the plaque psoriasis.