维A酸乳膏对煤焦油致兔耳痤疮模型的治疗作用

Tretinoin Cream Effect On The Acne Model Of Rabbit Ear Induced By Coal Tar

目的:探讨维A酸乳膏对煤焦油致兔耳痤疮模型的治疗作用。方法:18只雄性家兔随机分成正常对照组、模型组、维A酸乳膏组。模型组、维A酸乳膏组家兔内侧面耳管开口处2×2cm范围涂抹5%煤焦油,每日一次,每次0.25ml,连续14天造模。造模成功后,维A酸乳膏组造模处给予维A酸乳膏,0.25ml每天一次,连续14天;另两组不予处理。给药结束后,处死家兔,将耳部组织固定于10%福尔马林内作病理检查。结果:5%煤焦油连续涂抹14天,可观察到造模处毛囊明显增大,且毛囊口有明显角栓,痤疮样改变明显。给药结束后,病理检查显示,模型组角化过度,毛囊扩张,相邻毛囊融合,毛囊漏斗部扩大成壶状;维A酸乳膏治疗组轻度角化,毛囊扩张不明显,局部毛囊轻度扩张,未见相邻毛囊融合及毛囊漏斗部壶状扩大。结论:5%煤焦油可以引起兔耳粉刺样改变,维A酸乳膏对这种改变有明显的改善作用。

Objective： To Investigate the tretinoin cream effect on the acne model of rabbit ear induced by coal tar. Methods： 18 male rabbits were randomly divided into normal control group, model control group, Tretinoin cream group. 0. 25ml 5% coal tar is ap- plied to the inner surface of the rabbit ear once a day for 14 days. Then the rabbits of tretinoin cream group were treated by 0. 25ml tretinoin cream once a day for 14 days, others not. The ear tissue of all rabbits were fixed in 10% formalin for histological examination after two weeks. Results： Test area treated by 5% coal tar for 14 days can be observed that the hair follicles increased significantly and had obvious angle plugs. Histological examination show that hair follicle is hyperkeratosis, expansion and adjacent integration of the model group, and that hair follicle is mild hyperkeratosis, less expansion and adjacent not integration of the tretinoin cream group. Conclusion： This model is successful and which can be changed by tretinoin cream.