大鼠角膜碱烧伤后ILK和VEGF的表达与角膜新生血管的相关性研究

Relationship between corneal neovascu-larization and the expression of ILK and VEGF in cornea of rats after alkaline burn

目的:研究大鼠角膜碱烧伤后整合素连接激酶(ILK)、血管内皮生长因子(VEGF)的表达及其与角膜新生血管(CNV)的相关性。方法:通过碱烧伤Sprague-Dawley(SD)大鼠角膜制备角膜新生血管动物模型,采用形态学方法观察碱烧伤后新生血管的生成,免疫组织化学方法检测ILK及VEGF在碱烧伤后不同时间点的表达情况。结果:ILK及VEGF在正常大鼠角膜上皮中仅有微弱表达,而在碱烧伤后各时间点的角膜上皮以及血管内皮中均有明显表达(P<0.01)。碱烧伤后3～5d,可见二者阳性表达,且迅速增加;7～10d,二者表达达到高峰;14d以后二者表达逐渐减弱,与形态学观察CNV生长过程一致。即碱烧伤后3～5d新生血管生长迅速,7～10d生长达到高峰,14d以后血管开始逐渐减少退化。ILK及VEGF表达的变化均与CNV的变化呈正相关(ILK与CNV面积相关系数r=0.900,P<0.01;VEGF与CNV面积相关系数r=0.878,P<0.01),且二者之间也存在正相关性(r=0.926,P<0.01)。结论:大鼠角膜碱烧伤后ILK及VEGF均有明显表达,二者作为重要的调控子共同参与了CNV的形成过程。

AIM：To investigate the relationship between corneal neovascularization（CNV） and the expression of integrin-linked kinase（ILK） and vascular endothelial growth factor（VEGF） in cornea of rats after alkaline burn.METHODS：CNV model was established by alkaline cauterization of the central cornea of Sprague-Dawley（SD） rats.At the different time points,morphologic changes in CNV were observed with slit-lamp microscope,and the expression of ILK and VEGF in cornea was detected by immunohistochemical method and computer image analysis.RESULTS：ILK and VEGF were lowly expressed in epithelium of cornea in the control group,and strongly expressed in epithelium,endothelium and the endothelium cells of CNV in the experiment group（P0.01）.The expression of ILK and VEGF in cornea after alkaline burn,increased within the 3rd-5th day,reached the peak within the 7th-10th day,and decreased after the 14th day.The CNV in cornea after alkaline burn was found on the 3rd day,increased on the 5th day,reached the peak of area within the 7th-10th day,and decreased after the 14th day.The expression of ILK and VEGF showed positive correlation at different time points（r=0.926,P0.01）.And they were positively related to CNV（ILK and area of CNV,r=0.900,P0.01;VEGF and area of CNV,r=0.878,P0.01）.CONCLUSION：ILK and VEGF were expressed significantly in cornea of rats after alkaline burn.And they involved in the regulation of CNV.