ICR小鼠肥胖模型的建立以及肥胖指标和脂肪组织形态学比较

Establishment of the Obesity Model of ICR Mice and the Comparison of the Obesity Index and Morphology of Adipose Tissue

[目的]建立ICR小鼠肥胖模型,并比较肥胖型小鼠与对照组小鼠脂肪组织形态学的差异。[方法]以ICR小鼠为研究对象,饲喂高脂日粮构建肥胖模型,测定其体重、脂肪组织湿重和脂肪系数,并进行比较。[结果]雌性和雄性小鼠肥胖造模的成功率分别为16.67%和66.67%。用高脂日粮饲喂60 d后肥胖小鼠脂肪细胞体积明显增大,肥胖型小鼠体重、脂肪组织湿重和脂肪系数与对照组相比均存在极显著差异﹙P<0.01﹚。[结论]该研究为ICR小鼠肥胖模型的建立以及脂肪组织的形态学研究提供依据。

[Objective]The research aimed to establish the obesity model of ICR mice and compare the morphological differences of adipose tissue between obese mice and control mice.[Method]Taking ICR mice as the study objects,the obesity model was established by feeding high-fat diet.The body weight,wet weight of adipose tissue and fat coefficient of ICR mice were determined and the morphological comparison of adipose tissue was made.[Result]The success rate of the obesity model of female and male mice were 16.67% and 66.67% respectively.After ICR mice were fed with high-fat diet,the body weight,wet weight of adipose tissue and fat coefficient of obese mice had extremely significant difference with that in control group（P0.01﹚and the volume of adipose cells in adipose mice were obviously increased.[Conclusion]The research provided basis for the establishment of obesity model of ICR mice and the morphological study of adipose tissue.