甘草苷对T24细胞增殖和凋亡影响

Influence of Liquiritin on T24 Cell Proliferation and Apoptosis

目的:探讨甘草苷(Liquiritin,LQ)对人膀胱癌细胞T24增殖和凋亡的影响及其可能作用机制。方法:实验分为对照组、LQ20、50、100μg/mL组。MTT观察LQ对T24细胞增殖的影响,Annexin V/PI染色,流式细胞术检测LQ对T24细胞细胞凋亡的诱导作用,Western blot检测Caspase 3蛋白表达。结果:与对照组比较,LQ20、50、100μg/mL在24、48、72h均对T24有明显的细胞增殖抑制作用,LQ作用72h后细胞凋亡率增高,Casepase3蛋白含量明显增高。结论:LQ能抑制T24细胞的增殖和诱导细胞凋亡,其机制与Csaepase3蛋白表达增高有关。

Objective：The present study focuses on the effects of Liquiritin（LQ） on proliferation and apoptosis of human bladder cancer cell line T24 and its potential mechanism. Methods： Four groups including control, LQ20μg/mL, LQ50μg/mL and LQ100μg/mL groups were set up in this study. Cell proliferation was analyzed by MTY assay. Cell apoptosis of T24 induced by LQ was evaluated by flow cytometry with Annexin V / PI staining. Expression of Caspase 3 was detected by western blot. Results： Compared with the control, after treatment of LQ of 20μg/mL,50μg/mL, 100μg/mL for 24h ,48h ,72h, T24 cell proliferation decreased, OD value decreased,meanwhile,the apoptotic cells and Caspase 3 protein expression increased after treatment with LQ for 72h. Conclusion：The above results indicated that： LQ inhibited T24 cell proliferation, and also induced apoptosis, and these effects might be contributed to the increased Caspase 3 protein induced by LQ.