摘要
目的:研究姜黄素对血管瘤内皮细胞凋亡的影响。方法:在培养液体系中加入不同浓度的姜黄素进行不同时间长度的诱导,以MTT法检测细胞增殖,流式细胞术,TUNEL法检测细胞凋亡情况,Western blotting分析Bcl-2和Caspase-9蛋白表达。结果:1~50μmol/姜黄素均能抑制血管瘤内皮细胞增殖。5、10、50μmol/L姜黄素作用细胞4天后的凋亡率分别为(11.98±2.13)%、(17.96±1.25)%、(26.86±2.08)%,与对照组(4.03±1.16)%比较差异均有显著性意义(均P<0.05)。姜黄素浓度越高,诱导凋亡作用越明显,呈明显的剂量依赖效应。50μmol/L姜黄素作用细胞1、2、3、4天后的凋亡率分别为(6.93±1.06)%、(10.15±1.35)%、(17.21±2.06)%、(26.83±2.02)%,与对照组(3.98±1.03)%比较差异均有显著性意义(均P<0.05)。姜黄素作用时间越长,抑制血管瘤内皮细胞增殖作用和诱导凋亡的作用也越明显,呈明显的时间依赖效应。同时姜黄素浓度越大、作用时间越长,血管瘤内皮细胞细胞中Bcl-2蛋白的表达明显减少,而Caspase-9蛋白的表达明显增加。结论:姜黄素能够抑制血管瘤内皮细胞增殖、诱导血管瘤内皮细胞发生凋亡,并呈时间和剂量依赖效应,可能是通过下调凋亡抑制基因bcl-2表达,上调Caspase-9表达而实现的。
Objective : To investigate the effects of curcumin on inducing apoptosis of endothelial cells of angioma. Methods : Different coneent rations of eureumin were added into euhure media system to induce apoptosis of endothelial cells. The apoptosis was detected by MTF assay,flow eytometry and TUNEL method. Results:Curcumin at content rations of 1 -50p.mol/L could inhibit the proliferation . After the cells were treated with 5,10 and 50μmol/L curcumin for 4 days ,the rates of apoptosis were ( 12.46 ± 2. 37 )% , (18. 36± 1.32)% and(27.15 ± 2. 17 )% respectively, showing significant difference with that in control group (4. 09 ±1.18) % ,P 〈 0.05. The rates of apoptosis induced by 50μmol/L curcumin for 1,2,3 and 4 days were (7.35 ± 1. 28 )% , (10. 72 ± 1.68)%, (17.65 ± 2. 14)% and(27.15 ± 2. 17)% respectively ,significantly higher than in control group( 4.09 ± 1. 18) % ,P 〈 0.05, showing a timedependent manner. At the same time the concentration is more big and act time more longer, the expressions of Bcl -2s obviously reduced,while the expression of Caspase -9s obviously. Conclusion:Cureumin could induce apoptosis in a time and dose - dependent manner, which may be an approval to decrease expressions of bel - 2, and increade Caspase - 9.
出处
《辽宁中医杂志》
CAS
2012年第2期243-245,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
温州市科技局支持项目(20072345)