干扰素γ和γ射线对人肺癌A549细胞联合作用的体外观察

Combined effects of interferon γ and γ ray irradiation on A549 cells in vitro

目的 观察干扰素γ在人肺癌A549细胞放射治疗中的作用,探讨肺癌治疗的新途径.方法 单独γ射线辐照细胞或联合应用干扰素γ处理细胞,采用MTT法检测细胞存活率,Western印迹法分析细胞P53蛋白表达的变化,流式细胞仪测定细胞周期的变化.结果 与单独γ射线（5 Gy）作用相比,干扰素γ（ 1.0 U/ml）和γ射线联合应用能明显降低A549细胞的存活率（71.4％±2.1％比44.1％±3.1％,n=7,P＜0.01）；此外,与对照组相比,单独γ射线辐照组和单独干扰素γ给药组A549细胞P53蛋白表达水平有所升高（P＜0.05）,而干扰素γ和γ射线联合作用组细胞P53蛋白表达水平明显高于对照组（P＜0.01）；同时与单独γ射线治疗相比,联合治疗还能够显著影响细胞周期的变化,S期细胞增加（12.9％比20.9％,n=5,P＜ 0.05）,并引起G2/M期细胞阻滞（28.8％比38.9％,n=5,P＜ 0.05）.结论 干扰素γ和γ射线联合应用可以提高人肺癌A549细胞的放射治疗效果,在肺癌的治疗中可能具有一定的应用价值.

Objective To define the role of interferon-γon radiotherapy of lung cancer and explore a new way to clinical treatment.Methods A549 cells were exposed toγray with or without IFN-γ cotreatment.MTT assay was performed to evaluate cell viability. Western blot was used to observe the expression of P53 protein.Results The results showed that co-treatment of IFN-γ decreased the cell viability significantly compared with the γ ray irradiation group（71.4% ±2.1% vs 44.1% ±3.1%,n =7,P 〈 0.01 ）.In addition,the expression of P53 protein also increased significantly after co-treatment （P 〈0.01） ; Furthermore,the cell cycle was changed obviously in co-treatment group compared with γ ray irradiation group,S phase increased （ 12.9% vs 20.9%,n =5,P 〈 0.05 ） and also blocked the G2/M phase （28.8% vs 38.9%,n=5,P〈 0.05）.Conclusions The results suggested that γray irradiation combined with IFN-γ can increase the efficiency of radiotherapy on A549 cells and there is much broad prospect in the clinical treatment of lung cancer.