IL-1α和IL-1β在胃癌组织中的表达及其临床意义

Interleukin-1α and interleukin-1β in gastric cancer tissue and its clinical significance

目的:通过检测胃癌组织和癌旁组织中白细胞介素-1α(interleukin-1α,IL-1α)和IL-1β的表达水平并分析其与临床病理特征的关系,探讨IL-1α和IL-1β在胃癌发生、发展中的作用。方法:取73例原发性胃癌新鲜的肿瘤和对应远离肿瘤切缘(≥5 cm)的癌旁组织。用RIPA裂解液提取组织中的总蛋白,BCA法测定各组织的蛋白浓度,酶联免疫吸附实验检测组织中IL-1α和IL-1β的含量,细胞因子含量与蛋白浓度的比值为相对细胞因子含量,分析相对细胞因子含量与临床病理资料的关系。结果:胃癌组织中IL-1α的含量较癌旁组织明显升高[(3.450比2.665)ng/g,P<0.01],且表达水平随肿瘤增大、浸润加深而升高[(4.549比2.629)、(2.659比3.450)ng/g,P<0.05];尽管IL-1β的含量在肿瘤组织与癌旁组织中差异无统计学意义,但统计学分析显示IL-1β的含量随肿瘤增大而升高[(4.202比2.930)ng/g,P<0.05]。结论:炎性因子IL-1α和IL-1β在胃癌的发生发展中有重要作用。

Objective To investigate the association between the expression of interleukin（IL）-1α,IL-1β and the clinicopathologic parameters in gastric cancer,and to evaluate the significance of IL-1α and IL-1β in the development of gastric cancer.Methods Primary gastric tumor tissues and their corresponding adjacent nontumor tissues（≥ 5 cm away from the tumor） were collected from 73 patients with gastric cancer.The total protein was extracted by RIPA lysis buffer and protein concentration was determined by BCA method.Expressions of IL-1α and IL-1β in gastric cancer were measured by enzyme-linked immunosorbent assay.The association between relative levels of cytokines and clinicopathological characteristics was analyzed.Results The median level of IL-1α in gastric tissue was higher than that in adjacent nontumor tissue [（3.450 vs 2.665） ng/g protein,P0.01],and it was upregulated with the increase of tumor size and the depth of invasion [（4.549 vs 2.629）,（2.659 vs 3.450） ng/g protein,P0.05].Although no difference of the IL-1β level existed between tumor tissues and adjacent nontumor tissues,IL-1β levels were upregulated with the increase of tumor size [（4.202 vs 2.930） ng/g protein,P0.05].Conclusions Our results suggest that inflammatory cytokine IL-1α and IL-1β play important roles in the development of gastric cancer.