呼吸道合胞病毒毛细支气管炎与支气管哮喘的相关性研究

Relationship between respiratory syncytial virus bronchiolitis and bronchial asthma

目的探讨呼吸道合胞病毒(RSV)毛细支气管炎(毛支)与支气管哮喘两者发病机制的相关性。方法采用ELISA法检测31例RSV毛支患儿、25例支气管哮喘患儿、27例非RSV肺炎患儿和24例健康儿童外周血IFN-γ、IL-4、IL-10、TGF-β、IL-17水平,并进行比较分析。结果 RSV毛支患儿和哮喘患儿的IL-10、TGF-β水平显著低于非RSV肺炎患儿和健康对照儿童,而IL-4、IL-17水平则显著高于非RSV肺炎患儿和健康对照儿童(P均<0.05)。RSV毛支患儿和哮喘患儿的IFN-γ/IL-4、IL-10/IL-17比例显著低于非RSV肺炎患儿和健康对照儿童(P均<0.05),哮喘患儿的TGF-β/IL-17显著低于非RSV肺炎患儿与健康对照儿童(P均<0.05)。RSV毛支患儿与哮喘患儿之间、非RSV肺炎患儿与健康对照儿童之间IFN-γ、IL-4、IL-10、TGF-β、IL-17水平及其比值IFN-γ/IL-4、IL-10/IL-17、TGF-β/IL-17的差异均无统计学意义(P均>0.05)。结论 RSV毛支患儿与哮喘患儿存在相同的外周血细胞因子IFN-γ、IL-4、IL-10、TGF-β、IL-17水平的改变,这可能是其共同的发病机制之一。

Objective To explore the relationship between bronchiolitis caused by respiratory syncytial virus(RSV)and bronchial asthma.Methods The levels of interferon-γ(IFN-γ),interleukin-4(IL-4),interleukin-10(IL-10),transforming growth factor-β(TGF-β)and interleukin-17(IL-17)in 31 infants with RSV bronchiolitis,25 infants with asthma,27 infants with non-RSV pneumonia and 24 healthy infants were detected by ELISA.Results IL-10 and TGF-β levels in infants with RSV bronchiolitis and infants with asthma were significantly lower than those in the non-RSV pneumonia group and the normal control group(P < 0.05),while IL-4 and IL-17 levels were higher than those in the non-RSV pneumonia group and the normal control group(P < 0.05).The ratios of IFN-γ/IL-4 and IL-10/ IL-17 in infants with RSV bronchiolitis and infants with asthma were significantly lower than those in the non-RSV pneumonia group and the normal control group(P < 0.05),and the ratio of TGF-β/IL-17 in infants with asthma was significantly lower than that in the common pneumonia group and the normal control group(P < 0.05).No significant differences in the levels of IFN-γ,IL-4,IL-10,TGF-β and IL-17 and the ratios of IFN-γ/IL-4,IL-10/IL-17 and TGF-β/IL-17 were observed between the bronchiolitis group and the asthma group(P > 0.05),and also no significant differences were observed between the common pneumonia group and the normal control group(P > 0.05).Conclusions The same changes of the levels of IFN-γ,IL-4,IL-10,TGF-β and IL-17 in infants with RSV bronchiolitis and infants with asthma may be a common pathogenesis of RSV bronchiolitis and asthma.