肥胖大鼠血清环氧化酶-2水平变化及其与胰岛素抵抗的关系

The changes of serum cyclooxygenase-2 and its association with insulin resistance in obese rats

目的探讨肥胖大鼠不同病程中血清环氧化酶-2(COX-2)水平的变化及其与胰岛素抵抗的关系。方法将36只雄性SD大鼠随机分成肥胖组和对照组,分别于第14及20周末处死大鼠收集血清标本,检测各组大鼠血清COX-2、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和C反应蛋白(CRP)水平。结果第14和20周末,肥胖组大鼠血清COX-2、IL-6、TNF-α和CRP水平均显著高于相同周数对照组大鼠(P<0.01)。但不同周数肥胖组大鼠血清COX-2、IL-6、TNF-α和CRP水平比较差异无统计学意义(P>0.05)。Pearson直线相关分析显示,第14和20周末,肥胖组大鼠血清COX-2均分别与体重、内脏脂肪重量和CRP呈正相关性,与胰岛素敏感指数(ISI)呈负相关。结论不同病程中,肥胖大鼠血清中COX-2等炎症因子水平无明显变化,COX-2与肥胖及其相关的胰岛素抵抗密切相关。

Objective To investigate the changes o f serum cyclooxygenase-2（COX-2） level in different course and its association with insulin resistance in obese rats.Methods 36 Sprague-Dawlay male rats were randomly divided into two groups： the obese group and the control group.The blood sample was collected when the rats were killed at the end of the 14th and 20th week.The levels o f serum COX-2,IL-6,tumor necrosis factor-α（TNF-α） and C-reactive protein（CRP） were assayed.Results Both at the end of the 14th week and at the end of the 20th week,the levels of serum COX-2,IL-6,TNF-α and CRP were significantly higher in obese group than those in control group with the same week（P0.01）.But in different week,the levels of serum COX-2,IL-6,TNF-α and CRP had no statistic significance in obese group（P0.05）.Pearson linear correlation analysis showed that the level of serum COX-2 in obese group was positive correlation with body weight,viscera adipo weight and CRP,and inverse correlation with insulin sensitivity index（ISI）.Conclusion The levels of inflammatory factors such as COX-2 did not change significantly in different course,COX-2 played an important role in obesity and insulin resistance related to obesity.