羰酰氰间氯苯腙对多重耐药大肠埃希菌最低抑菌浓度的影响

The influence of energy inhibitor cccp on the MIC of multidrug resistant Escherichia coli

目的了解本地大肠埃希菌(Escherichia coli)对6种常用抗菌药物的药敏情况以及碳酰氰间氯苯腙(CCCP)对多重耐药大肠埃希菌最低抑菌浓度(MIC)的影响。方法纸片扩散法(K-B法)进行6种抗菌药物的药物敏感性检测;PCR技术检测受试菌株携带acra、acrb基因的情况;常量肉汤稀释法测定大肠埃希菌对两种氟喹诺酮类抗菌药物的MIC,并对比加入CCCP后MIC的变化。结果 71株大肠埃希菌对三类6种抗生素耐药结果中环丙沙星(CIP)耐药率最高52株(73.24%),氨曲南(ATM)最小30.99%,耐药模式以多药耐药为主(51.11%);外排泵基因acra,acrb在多重耐药菌中阳性率高达91.89%和81.03%;加入CCCP前后多重耐菌株和敏感株的MIC值的变化差值经秩和检验差异有统计学意义。结论本地大肠埃希菌存在明显的多重耐药现象;外排泵基因acra和acrb是大肠埃希菌多药耐药的重要原因之一;主动外排泵抑制剂CCCP能够降低氟喹诺酮类抗菌药物对多耐药大肠埃希菌的MIC。

Objective To investigate the resistant situation of local Escherichia coli,and research on the influence of MIC which carbonylcyanidem-chlorophenyl hydrazone（CCCP） associate wtih two fluoroquinolones.Methods The susceptibility of 71 strains of E.coli to 6 antibiotics were detected by disk diffusion;Acra,acrb were detected by PCR;The MIC of Escherichia to two fluoroquinolones wre tested by Constant broth dilution method,and compare the variation of MIC which CCCP has been added.Results The resistant rates of Escherichia coli to CIP is the highest.73.24%,but ATM is the lowest 30.99%.Multidrug resistance was the main part of resistant patterns（51.11%）.Acra/acrb positive rate is the highest in multidrug resistantthe.Variation of MIC in resistant and sensitive strains had statistical difference with cccp.Conclusion The multidrug resistance is obivous.Acra and acrb are important reason of multidrug reasistance.Efflux system inhibitors CCCP combine with fluoroquinolone antibiotics will reduce MIC.