青蒿素衍生物对非小细胞肺癌A549细胞增殖的影响

Inhibition effect of Artemisinin and Its Derivatives on A549 Cells

目的:观察青蒿素衍生物对非小细胞肺癌A549增殖的影响。方法:通过MTT法初步对青蒿素、双氢青蒿素和青蒿琥酯进行TC50的测定,选择低毒剂量TC10作为实验浓度,再以动态MTT法观察各青蒿素衍生物在低毒剂量下对非小细胞肺癌A549增殖的影响。结果:青蒿素、双氢青蒿素、青蒿琥酯的TC50分别为1.77 mmol/L、58.24μmol/L、64.33μmol/L;在对A549细胞增殖影响中,双氢青蒿素和青蒿琥酯能延长细胞倍增时间,减缓细胞增殖周期,但青蒿素对细胞倍增时间没有影响,仅能延长细胞到达高峰期的时间。结论:青蒿素衍生物中,青蒿素抗肿瘤作用稍弱,青蒿琥酯和双氢青蒿素抗肿瘤作用较好,能同时延长细胞倍增时间和增殖周期,显示对肺癌A549细胞的增殖有直接抑制作用。

Objective： To observe the inhibitory effect of artemisinin and its derivatives on non-small cell lung cancer A549 cells.Methods： The TC50（concentration required to cause 50% toxicity compared with controls）of artemisinin,dihydroartemisinin,and artesunate on A549 cells was detemined by MTT assay.The TC10 was selected as the experimental dosage in the proliferation inhibition of A549 assay.Results： The TC50 of artemisinin,dihydroartemisinin,and artesunate were 1.77 mmol/L,58.24 μmol/L,64.33 μmol/L,respectively.Artemisinin had no direct inhibition effect on the proliferation of A549,but dihydroartemisinin and artesunate prolonged the the cell cycle progression by delaying the double proliferation time.Conclusion： Dihydroartemisinin and artesunate may have direct inhibitory effect on the proliferation of A549 cells.