摘要
目的建立甲硝唑片溶出度的高效液相测定方法。方法色谱柱为Waters Sunfire CI8柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(20∶80),流速为1.0 ml.min-1,检测波长为320 nm,柱温为30℃。结果甲硝唑在0.05~0.90 g.L-1浓度范围内呈良好线性关系(r=1.0),平均回收率为100.2%(n=9),RSD为0.39%。结论该方法准确、可靠,可用于甲硝唑片的溶出度测定。
Objective To establish an HPLC method for dissolution of metronidazole tablets.Methods Waters Sunfire CI8 column(4.6 mm×150 mm,5 μm)was used with a mobile phase composed of methanol-water(20∶ 80)at the flow rate of 1.0 ml·min-1.The detection wavelength was 320 nm and the temperature of the column was 30℃.Results The calibration curve of Metronidazole was linear in the range of 0.05~0.90 g·L-1(r=1.0).The average recovery was 100.2% with an RSD of 0.39%(n=9).Conclusion This method is accurate,reliable and can be used for the dissolution of metronidazole tablets.
出处
《安徽医药》
CAS
2012年第1期38-39,共2页
Anhui Medical and Pharmaceutical Journal