苍耳子配伍黄芪的HPLC-DAD指纹图谱研究

Study of Variation on Fingerprint Chromatogram of Compatibility Extract Derived from Fructus Xanthii and Radix Astragali by HPLC-DAD

目的建立苍耳子配伍黄芪的高效液相色谱(HPLC)指纹图谱分析方法,研究配伍对各单味药主要特征峰的影响。方法采用HPLC分析苍耳子、黄芪单煎及苍耳子配伍黄芪合煎样品,选用Diamonsil C18色谱柱(5μm,250 mm×4.6 mm),以甲醇-0.2%甲酸水为流动相进行线性梯度洗脱,流量为0.8 mL/min,检测波长为260 nm。结果建立了苍耳子配伍黄芪的HPLC指纹图谱,基本为两单味药特征峰的加和,无明显新特征峰的增加,但配伍合煎对某些成分的溶出有明显的相互抑制作用。结论本方法简便、快捷,为临床类方的配伍应用以及制定复方质量标准提供了参考。

Objective To establish the method for analyzing compatibility components from Fructus Xanthii and Radix Astragali by HPLC fingerprint chromatogram and to study the influence of main characteristic peaks variations induced by compatibilities.Methods Diamonsil-C18 column（4.6 mm×250 mm,5 μm） was used to analyse these samples including the water extracts of Fructus Xanthii,Radix Astragali and compatibilities between them,with methanol and water containing 0.2% methanoic acid as mobile phases in a gradient elution,detection wavelength as 260 nm,and flow rate was 0.8 mL/min.Results HPLC fingerprint chromatogram of the compatibilities from Fructus Xanthii and Radix Astragali was established,and the whole characteristic peaks were the summation,with no new characteristic peaks existing.Compared with the single decoction,dissolution rate of some ingredients was decreased reciprocally in compatibilities.Conclusion The method is simple and convenient,which provides the references to clinical applications of compatibility and the basis for the quality assessment of compound.