苦参碱对人肺腺癌A549细胞增殖及HIF-1α、VEGF表达的影响

Effect of matrine on proliferation and HIF-1α,VEGF expression of A549 cells

目的探讨苦参碱对人肺腺癌A549细胞增殖的影响及作用机制。方法将0、5、50、100、250、500μg/mL质量浓度的苦参碱分别作用于体外培养的处于对数生长期的A549细胞,MTT法检测细胞生长抑制率,RT-PCR法检测细胞缺氧诱导因子-1α(HIF-1α)mRNA和血管内皮生长因子(VEGF)mRNA表达。结果苦参碱呈时间、剂量依赖性抑制A549细胞生长(P<0.05);呈时间、剂量依赖性降低HIF-1α、VEGF mRNA表达(P<0.05);HIF-1α和VEGF之间表达呈正相关(r=0.994,P<0.01)。结论苦参碱能抑制人肺腺癌A549细胞的增殖,其机制可能为降低HIF-1α和VEGF表达,从而抑制肺癌组织血管生成。

Objective To explore the effect and mechanism of matrine on the proliferation of A549 cells. Methods A549 cell line in logarithmic growth cultured in vitro was treated by matrine with the concentration of 0, 5, 50, 100,250, 500 μg/mL, respectively, and then cellular proliferation was evaluated by MTT method. Hypoxia-inducible factor-1 α（ HIF- 1 α ）and vascular endothelial growth factor （VEGF） mRNA expression in A549 cells were detected by RT-PCR analysis. Results Matrine inhibited the proliferation of A549 cells in a dose and time-dependent manner（ P 〈 0.05 ） ; inhibited the expression of HIF-1α, VEGF mRNA in a close and time-dependent （P 〈 0. 05 ） ; there was a positive correlation between the expression of HIF-1α mRNA and VEGF mRNA（ r = 0. 994 ,P 〈 0. 01 ）. Conciusion Matrine can inhibit the proliferation of A549 cel1 line, the mechanism may down-regulate the expression of HIF-1α and VEGF, so to inhibit the vascular growth of lung cancer tissue.