中药补脑止痫散对癫痫大鼠海马神经元凋亡基因Bax和Bcl-2表达的影响

Effect of Bunaozhixian Powder on neuronal apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats

目的通过观察中药补脑止痫散对戊四氮化学点燃癫痫模型大鼠海马神经元凋亡基因Bax和Bcl-2表达的影响,探讨补脑止痫散治疗癫痫的相关机制。方法从78只正常Wistar大鼠中随机选取10只列为正常组,剩余68只大鼠腹腔注射戊四氮制作癫痫点燃模型,再随机选取50只造模成功大鼠随机分为5组:模型组、丙戊酸钠组、补脑止痫散高剂量组、补脑止痫散中剂量组和补脑止痫散低剂量组,每组10只。用免疫组化法检测药物治疗各组大鼠与正常组和模型组比较海马中神经元凋亡基因Bax和Bcl-2蛋白表达的变化。结果各治疗组大鼠海马Bax和Bcl-2蛋白阳性目标面密度值与模型组相比分别出现明显降低和升高(P<0.05),而补脑止痫散低剂量组大鼠海马Bax和Bcl-2蛋白阳性目标面密度值改变不明显(P>0.05),并且补脑止痫散高剂量组和中剂量组比较,以及此二组与丙戊酸钠组相比大鼠海马Bax和Bcl-2蛋白阳性目标面密度值改变均无明显差异(P>0.05)。结论中药补脑止痫散高、中剂量能调整癫痫患者海马神经元凋亡基因Bax和Bcl-2蛋白的表达,从而抑制癫痫发作。

Objective To explore the mechanism of the Bunaozhixian Powder on treatment of epilepsy,we observe the effect of Bunaozhixian Powder on neuron apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats induced by pentylenetetrazol（PTZ）.Methods Select 10 rats from 78 normal wistar rats randomly as the normal group.The remainder 68 rats are injected abdominally with PTZ as epileptic model.Then randomly select 50 epileptic rats,divide these rats into 5 groups： the model group、the sodium valproate group、high dosage of Bunaozhixian Powder group、medium dosage of Bunaozhixian Powder group and low dosage of Bunaozhixian Powder group.Every group has 10 rats.5 groups are injected with PTZ as epileptic model except the normal group.Examine the hippocampus cerebral tissues Bax、Bcl-2 protein expression with enzyme immunoassay method in every group.Results Compared with the model group,the positive Bax and Bcl-2 protein density value of hippocampus neuron area of rats with medical treatment get significant lower and higher respectively（P0.05）,but low dosage of Bunaozhixian Powder group have no significant change（P0.05）.Besides,high dosage is compared to medium dosage of Bunaozhixian Powder group,and these two groups are compared to the sodium valproate group,the positive Bax and Bcl-2 protein density value have no significant change（P0.05）.Conclusion The high and medium dosage of Bunaozhixian Powder can change the neuronal apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats.So the Bunaozhixian Powder can inhibit epileptic seizures.