Wnt3a影响大鼠海马神经干细胞增殖的体外研究

The effect of Wnt3a on the proliferation of neural stem cells in vitro

目的:探讨Wnt3a对胚胎大鼠海马神经干细胞(neural stem cells,NSCs)体外增殖的影响。方法:采用机械分离、无血清传代培养法,从胎鼠海马中获得NSCs,使用免疫荧光法对NSCs及其分化情况进行鉴定。将NSCs与5-溴脱氧尿嘧啶核苷(Brdu)共孵育,观察Wnt3a对NSCs体外增殖情况的影响。结果:海马源胚胎NSCs具有增殖能力,可以传代培养,可分化为神经元和星形胶质细胞。与Brdu共孵育后,添加Wnt3a的实验组Brdu阳性率高于对照组(P<0.01)。结论:使用机械分离、无血清培养的方法获得的NSCs具有增殖和多向分化能力,Wnt3a可以促进大鼠海马NSCs的体外增殖。

Objective：To investigate the effect of Wnt3a on the proliferation of neural stem cells（NSCs）.Methods：NSCs were separated mechanically from hippocampus and cultured in serum-free medium.Immunofluorescence technique was employed to detect nestin antigen which was specific antigen of mature neural cells.NSCs were co-incubated with 5-bromodeoxyuridine（Brdu） and immunofluorescence staining was used to observe proliferation potential.Results：The NSCs isolated from hippocampus of embryonic rats the proliferative ability,and differentiated into neurons and astrocytes.The positive rate of the cells stained with Brdu in experimental group was significantly higher than that in the control group（P0.01）.Conclusions：NSCs isolated from hippocampus possessed the ability of proliferation and pluripotent differentiation.Wnt3a can promote the proliferation of neural stem cells.