绿豆基因组SSR引物在豇豆属作物中的通用性

Transferability of Mungbean Genomic-SSR Markers in Other Vigna Species

分子标记的种间通用性可降低其开发成本,提高利用效率,也有助于促进遗传研究较薄弱物种的分子遗传学研究。本文选取绿豆、小豆、豇豆及饭豆材料各3份,分析1205对新开发的绿豆基因组SSR引物在这些材料中的扩增效果,结果显示绿豆基因组SSR引物在豇豆、小豆和饭豆中的通用性比率分别为50.0%、73.3%和81.6%;多态性比率分别为4.1%、1.7%和1.5%;在4个种间均通用的引物469对。这些通用性SSR引物将有助于这4种食用豆类在多样性评价、连锁图谱的构建、基因定位及比较基因组学等方面的研究。

Cowpea（Vigna unguiculata L.Walp.）,mungbean（V.radiata L.Wilczek）,rice bean [V.umbellate（Thunb.） Ohwi Ohashi],and adzuki bean（V.angularis Willd.Ohwi and Ohashi） are major food legumes in China.At present,SSR markers for genetic analysis of these legumes are much limited.Transferability analysis of primers has the vital significance to reduce their development cost and improve their development efficiency.In this study,1 205 SSR primers were tested for their transferability and polymorphism by PCR amplification with the genomic DNA of four Vigna species,cowpea,adzuki bean,mungbean and rice bean.The results indicated that the transferability rate of mungbean genomic-SSR in cowpea,adzuki bean and rice bean was 50.0%,73.3%,and 81.6%,and the ratio of polymorphism SSR primers in these crops was 4.1%,1.7%,and 1.5%,whereas 32.0% in mungbean.A total of 469 mungbean genomic-SSR primers were detected to be highly transferable among different species of Vigna.The transferability of mungbean genomic-SSR was higher in adzuki bean and rice bean than in cowpea.These transferable markers are useful for further genetic and breeding studies in these species.