肝癌组织血管内皮生长因子与受体的表达及其临床意义

Expressions of VEGF and its receptor in primary hepatocellular carcinoma,and their clinical significance

目的研究血管内皮生长因子(VEGF)及受体(VEGFR)在肝癌组织中的表达及其临床意义。方法采用免疫组化SP法检测76例肝癌切除标本中VEGF、VEGFR的表达,分析VEGF、VEGFR与肝癌临床发病特征、术后生存时间、术后复发的关系。结果本组76例1年后复发29例、2年后复发16例,累计1、2年平均无瘤生存率分别为61.85%±5.57%、40.97%±5.63%;76例肝癌手术标本中VEGF阳性表达61例,阳性率80.3%,VEGFR阳性表达67例,阳性率88.2%,并且统计发现VEGF、VEGFR的阳性表达和肿瘤的大小、数目、有无包膜、术前是否肝内转移都是影响肝癌术后复发与生存率的关键因素,而VEGF及VEGFR的阳性表达与肿瘤体积大小、有无包膜、术前是否转移更是密切相关;VEGF、VEGFR的表达二者之间也相互关联。结论 VEGF、VEGFR过度表达与肝癌术后复发及生存时间密切相关,联合分析二者的表达对肝癌手术治疗的预后判断有更大指导价值。

Objective To study the expressions and their clinical significance of vascular endothelial growth factor （VEGF） and its receptor （VEGFR） in surgical specimens from patients with primary hepatocellular carcinoma （HCC）. Methods Seventy-six cases of HCC patients underwent tumor resection were analyzed. The tumor biopsy samples were detected by SP immunohistochemistry. Expression intensities of VEGF,VEGFR were qualitatively analyzed. The survival time and recurrence and metastasis were followed up for over 2 years after resection. Relationship between the expressions of VEGF,VEGFR and the prognosis were analyzed. Results Among the 76 cases 29 cases recurred after 1 year,16 cases recurred after 2 years, which their tumor-free survival rate was 61.85%＋5.57%, 40.97%＋5.63% respectively; positive rate of VEGF and VEGFR in primary HCC was 80.3%（61/ 76） and 88.2%（67/76） respectively. The factors affecting survival time were metastasis, number of primary focus, capsule, tumor size and the expression of VEGF, VEGFR. And the expressions of VEGF and VEGFR were closely correlated with tumor size, capsule, preoperative metastasis. The expressions of VEGF, VEGFR were related each other. Conclusion VEGF and VEGFR overexpressions are closely correlated with recurrence and survival time of HCC patient, and they can be served as important index in monitoring the prognosis of HCC after resection.