丹红注射液预处理对大鼠缺血再灌注心肌细胞损伤的影响

The effect of Danhong Injection pretreatment on cardiomyocytes cell of ischemic-reperfusion rats

目的研究丹红注射液预处理对大鼠心肌细胞缺血再灌注损伤的作用。方法体外培养大鼠心肌细胞,将丹红注射液以2%、4%、8%、16%四种浓度分别加入培养基中,预处理24 h后,置于缺血再灌注(I/R)环境中培养,采用流式细胞仪技术检测各组细胞的凋亡率,免疫组织化学法检测凋亡基因Bax及Bcl-2蛋白表达变化。结果与正常对照组比较,缺血再灌组细胞凋亡率及Bas表达明显升高,Bcl-2表达则显著降低;而用丹红注射液预处理后呈浓度依赖性地下调凋亡率及Bas表达,上调Bcl-2表达。结论丹红注射液对缺血再灌心肌细胞有保护作用,且与浓度有一定的依赖关系,其作用可能是通过上调Bcl-2蛋白表达,抑制Bax蛋白表达实现的。

Objective To investigate the effect of Danhong Injection pretreatment on cardiomyocytes cell of ischemic-reperfusion rats.Methods The rats cardiomyocytes were cultured and pretreated with Danhong Injection in four different concentrations： 2%,4%,8%,16% before create cardiomyocytes I/R model after 24 hours for pretreatment.The apoptotic rate of cardiomyocytes was determined by flow cytometry,the protein expressions of Bcl-2 and Bax were observed by imnmnohistochemistry.Results Compared with control group,the apoptotic rates were significantly increased in ischemia-reperfusion（I/R） group,and the protein expressions of Bax expressions was significantly up-regulated and Bcl-2 expressions was down-regulated.Pretreatment of cardiomyocytes with Danhong Injection increased the expression of Bcl-2,decreased the expression of Bax and the apoptotic rate in a dose-dependent fashion.Conclusion Danhong Injection alleviates I/R injury in a concentration-dependent manner.These effects may be partially that Danhong Injection can inhibit Bas gene protein expression and induce Bcl-2 gene protein expression.