参七粉对动脉粥样硬化家兔血脂代谢及血管内皮功能的影响

Influence of Shenqi Powders on lipid metabolism and vascular endothelial function in rabbits with atherosclerosis

目的观察参七粉对动脉粥样硬化(AS)家兔模型血脂代谢和血管内皮功能的影响,探讨参七粉抗AS作用及可能机制。方法将40只健康新西兰家兔随机分为对照组、模型组、参七粉组和血脂康组,每组10只,分别给予普通饲料、高脂饲料、高脂饲料加参七粉、高脂饲料加血脂康饲喂。各组于实验开始及实验第8周末分别在清晨空腹由兔耳静脉采血,测血脂、血栓素B2(TXB2)、6-酮-前列环素(6-K-PGF1α)、内皮素(ET),并于8周后处死动物取主动脉作病理形态学观察。结果与对照组比较,模型组、参七粉组和血脂康组血清血脂、血浆TXB2、ET均显著升高(P<0.05或P<0.01),6-K-PGF1α/TXB2比值显著下降(P<0.05或P<0.01);与模型组比较,参七粉组和血脂康组均能显著降低模型动物血脂水平(P<0.05)和血浆TXB2、ET(P<0.05),改善6-K-PGF1α/TXB2比值(P<0.05)。病理结果显示,参七粉组和血脂康组主动脉粥样硬化病变及血管内皮细胞损伤明显轻于高脂模型组。结论参七粉具有明显的抗动脉粥样硬化作用,其作用机制可能与调节脂质代谢、影响血管内皮细胞活性的活性物质生成有关。

Objective To observe the effects of Shenqi Powders on lipid metabolism and vascular endothelial function in rabbits with atherosclerosis and to explore its possible mechanism.Methods Forty healthy New Zealand rabbits were randomly divided into 4 groups（n = 10）： normal control group（fed with normal diet）,hyper-cholesterol model group（fed with high cholesteroldiet）,Shenqi Powders group（fed with high cholesterol and Shenqi Powders diet） and Xuezhikang group（fed with high cholesterol and Xuezhikang）.After 8 weeks,serum lipids,plasma TXB2,6-K-PGF1α and ET were detected in each group,and rabbits were killed and the aortas were observed by the microscope.Results Compared with normal control group,the levels of serum TC,TG,LDL-C and plasma TXB2 and ET significantly increased（P 〈0.05 or P〈 0.01）,the levels of 6-K-PGF1α/TXB2 markedly decreased in hyper-cholesterol model group,Shenqi Powders group and Xuezhikang group;compared with hyper-cholesterol model group,the levels of serum TC,TG,LDL-C and plasma TXB2 and ET significantly decreased（P〈 0.05）,the levels of 6-K-PGF1α/TXB2 markedly improved in Shenqi Powders group and Xuezhikang group;pathological results showed that,atherosclerosis lesions and the structural injuries of vascular endothelial cells in Shenqi Powders group were milder than that in hyper-cholesterol model group.Conclusion The Shenqi Powders has good effect against atherosclerosis,the possible mechanism may be related to regulation of lipid metabolism and the protection of the functions of vascular endothelial cells.