空肠弯曲菌肠毒素产生最佳培养条件的研究

Study of the Best Culture Conditions for Campylobacter jejuni Enterotoxin Production

通过对Ｃ．ｊｅｊｕｎｉ产生ＣＥ的最佳培养基及多粘菌素Ｂ、Ｆｅ３＋ 、培养温度、培养时间、传代次数影响ＣＥ 产生的研究，确定了Ｍ１９９＋ Ｔ（胰蛋白胨１０ｇ／Ｌ）＋ ＡＡ（２．５ｇ／Ｌ的Ｌ－丝氨酸、Ｌ－谷氨酸）＋ ＦｅＣＬ３（０．７５ｍ ｇ／Ｌ）是产生ＣＥ的最佳培养基，３７℃微需氧培养４８ｈ 是产生ＣＥ的最佳培养条件。同时，还发现Ｃ．ｊｅｊｕｎｉ传代５ 次以上，产生ＣＥ的能力下降，因此用于ＣＥ检测的菌株传代不得超过４ 代。

The best medium for the production of C.jejuni CE and the influence of polymyxin B,Fe 3+ ,culture temperature and hours,passaged times of the bacteria on CE production were studied in this paper.It was showed that Medium 199 plus 10 g/L tryptone, 2.5 g/L L serine and L glutamic acid,0.75 mg/L ferric chloride(M 199+T+AA+Fe 3+ )was the best medium for the production of C E,and that the bacteria inoculated in M 199+T+AA+Fe 3+ and incubated at 37℃ for 48 h in a microaerophilic atmospher was the best condition for the production of CE.It was also found that the bacteria passaged more than 5 times would reduce the output of CE.Therefore,the bacteria detected for CE could be passaged no more than 4 times