摘要
从土壤中分离出1株可产抗真菌蛋白的菌株,通过形态观察和分子生物学实验等方法鉴定其为巨大曲霉,编号AF-11。研究证明,利用纤维素阳离子交换柱层析法和凝胶柱层析法可从该菌的发酵上清液中分离纯化出高活性的抗真菌蛋白(AFP),该蛋白对青霉属、曲霉属和镰刀霉属等产毒丝状真菌的生长具有明显的抑制作用。
A strain which can produce antifungal protein was isolated from soil and was identified as Aspergillus giganteus,numbered AF-11,through morphologic observation and molecular biology experiments.It has been proven that the high activity and purity antifungal protein（AFP） which can obviously inhibit the growth of filamentous fungi such as Penicillium,Aspergillus and Fusarium,can be separated from the fermentation supernatant by using cellulose cation exchange column chromatography and gelatin column chromatography.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第1期57-61,共5页
Food and Fermentation Industries
关键词
巨大曲霉
抗真菌蛋白
鉴定
提纯
Aspergillus giganteus
antifungal protein
ddentification
purification
