高温蒸煮预处理甘蔗叶制备木聚糖

Study on Preparation of Xylan from High-temperature Boliled Pretreated Sugarcane Leaves

研究了高温蒸煮预处理甘蔗叶制备木聚糖的工艺过程,探究预浸方式、液固比、蒸煮时间、蒸煮温度4个因素对蒸煮结果的影响。研究表明:蒸煮液中总糖含量随蒸煮温度升高而增大;随蒸煮时间延长,先增大后减小;随液固比增大,先增高后降低。选用的最适工艺条件:用水将甘蔗叶在60℃下浸泡12 h,液固比是10∶1,浸泡液在126℃下密封蒸煮60 min。同时使用电子扫描显微镜分析蒸煮前后蒸煮渣的形态,表明高温蒸煮有利于木聚糖的溶出。

This paper mainly studies the high-temperature steamed sugarcane leaves For preparing xylan.There were four factors influenced extraction： pre-soaking methods,liquid-solid ratio,boiling time and temperature.The results show that： when the boiling temperature rose,the total sugar content increased;when boiling time extend,it increased firstly,then reduced;When the proportion of water to the sugarcane leaves increased,it increased and then decreased.This study also provided the best production technology conditions： the sugarcane leaves soaking for 12 h with water at 60 ℃,for 60 min,the proportion of the sugarcane leaves with distilled water 1∶ 10,then cooking at 126 ℃ for 60minutes.Scanning electron microscope（SEM） analysis was used to observe residue morphology before and after cooking.The results indicated that high-temperature cooking helped the dissolution of xylan.This result provided a basis to prepare xylo-oligosaccharides from xylan by enzyme in future.