防制胚泡着床障碍中药的筛选及药效研究

Screen and Pharmacodynamic Action of Chinese Materia Medica for Prevention and Cure Embryo Implantation Dysfunction

为了筛选防制胚泡着床障碍中药,本试验利用皮下注射米非司酮所致的小鼠胚泡着床障碍模型,以妊娠率、着床胚泡数、雌激素和孕激素及其比值为指标,将丹参、黄芪、川芎、黄芩、白术、菟丝子、桑寄生、续断、党参、当归等中药组成复方进行筛选和药效评价。筛选试验结果表明,方3组妊娠率显著高于方1组、菟丝子组和续断组(P<0.05),也高于方2组和对照组,说明方3有利于小鼠胚泡的着床。方3的药效试验表明,保胎组妊娠率极显著高于对照组和高剂量组(P<0.01),显著高于低剂量组(P<0.05)。保胎组孕酮水平、孕酮和雌二醇比值极显著高于高、低剂量组、对照组和正常组(P<0.01),孕酮和雌二醇比值显著高于中剂量组(P<0.05),而保胎组、低剂量组和正常组雌二醇水平显著低于高剂量组和对照组(P<0.05),中剂量组孕酮水平显著高于对照组(P<0.05)。这表明方3能提高小鼠的孕酮水平及孕酮和雌二醇比值,有利于小鼠胚泡着床。

To screen Chinese materia medica for prevention and cure embryo implantation dysfunction,mouse model with embryo implantation dysfunction was established by injected subcutaneously with mifepristone.Complex prescription composed of such Chinese materia medica as Salvia miltiorrhiza,Radix astragali,Szechwan lovage rhizome,Radix scutellariae,Rhizoma atractylodis macrocephalae,Semen cuscutae,Herba taxilli,Radix dipsaci,Radix codonopsis,Radix angelicae sinensis and some of them were evaluated in the model,pregnancy rate（PR）,number of embryo implantation,progestin（P4）,estradiol（E2） and ratio of progestin to estradiol（P4/E2） were the main index.The results of the screen test showed that PR was significantly higher in prescription 3 group than that in prescription 1 group,Semen cuscutae group and Radix dipsaci group（P0.05）,and was higher than those in the prescription 2 group and control group,which indicated that prescription 3 was benefit to embryo implantation in mice.Pharmacodynamic test showed that PR was extreme significantly higher in tocolysis group than those in the control group and high-dose group（P0.01）,and was significantly higher than that in the low-dose group（P0.05）.Serum P4 level and P4/E2 were extreme significantly higher in the tocolysis group than those in the high-dose group,low-dose group,control group and health group（P0.01）,and P4/E2 was significantly higher in the tocolysis group than that in middle-dose group（P0.05）,while E2 level in serum was significantly lower in the tocolysis group,low-dose group and health group than that in the high-dose group and the control group（P0.05）.P4 level in serum was significantly higher in the middle-dose group than that in the control group（P0.05）.In conclusion,prescription 3 could improve progestin level in serum and the ratio of progestin to estradiol in mice,which were helpful for embryo implantation.