不同脱钙液对兔膝关节组织切片染色的影响

The Influence of Different Decalcifying Fluid on the Section Staining of Articular Joint Tissue of Rabbit Knee

探讨不同脱钙液对新西兰大白兔膝关节切片染色效果的影响,为膝关节关节软骨及基质多糖的观察奠定试验基础。取正常新西兰大白兔膝关节,10%中性福尔马林溶液充分固定,流水冲洗后,分别浸入10%硝酸脱钙液、3%硝酸脱钙液、改良AF硝酸脱钙液及改良30%盐酸脱钙液中进行充分脱钙后,流水冲洗,制作石蜡切片,苏木精—伊红(HE)、奥尔辛蓝及沙红O染色,显微镜检观察并评价其效果。结果表明:①HE染色效果,3%硝酸组>30%盐酸组>10%硝酸组及改良AF硝酸组;②奥尔辛蓝及沙红O染色效果,3%硝酸组及改良AF硝酸组优于其他各组。

To investigate the effect of different decalcifying fluid on the color of articular joint tissue section of rabbit knee,to aid at the histological evaluation of hyaline cartilage and distribution of fibrocartilage polysaccharide.Prepare New Zealand rabbits,after the fully fixation of the whole rabbit knee joints in 10% neutral formalin,the tissues were washed with running water,then decalcified with 4 different decalcifying solutions（10% HNO3 decalcifying solutions,3% HNO3 decalcifying solutions,modified AF decalcifying solutions and modified 30% hydrochloric acid）.The tissue sections were stained with hematoxylin-eosin（HE）,alcian blue and safranin-O,and observed under a microscope to evaluate the morphology of chondrocytes and the cartilage matrix.Results：①The effects of HE staining： first is 3% HNO3 decalcifying solutions group,next is modified 30% hydrochloric acid group,once again is 10% HNO3 decalcifying solutions and modified AF decalcifying solutions group.②The effect of alcian blue and safranin-O staining in 3% HNO3 decalcifying solutions group and modified AF decalcifying solutions group were better than other groups.