响应面法优化微波辅助提取枳壳中总黄酮工艺

Optimization of Microwave-Assisted Extraction of Total Flavonoids from Fructus Aurantii Immaturus by Response Surface Methodology

利用响应面法对枳壳中总黄酮的微波提取工艺条件进行优化。在单因素试验基础上,利用中心组合设计原理及响应面法分析建立二次回归模型。以微波功率、提取时间、乙醇体积分数为自变量,柚皮苷、橙皮苷得率的综合指标为响应值,研究各因素及其交互作用对总黄酮得率的影响。利用模型的响应曲面图及其等高线图,确定微波提取枳壳中总黄酮的最佳工艺参数为枳壳粉碎颗粒度为40～60目、液料比100:1(mL/g)、乙醇体积分数58.8%、微波功率447W、提取时间3.9min,在此条件下,柚皮苷、橙皮苷的提取得率分别达到50.21、4.96mg/g,与理论预测值基本吻合。

This paper deals with the optimization of the process conditions for the microwave-assisted extraction of total flavonoids from Fructus Aurantii Immaturus using response surface methodology（RSM）.Based on one-factor-at-a-time experiments,a quadratic regression model indicating the individual and interactive effects of microwave power,extraction time and ethanol concentration on the yields of naringin and hesperidin was established using a Box-Behnken experimental design.From the response surface and contour plots drawn for the model,the optimal extraction conditions were obtained as follows： size reduction ratio 40-60 mesh,liquid/solid ratio 100：1,ethanol concentration 58.8%（V/V）,microwave power 447 W,and extraction time 3.9 min.Under these conditions,the experimental yields of naringin and hesperidin were 50.21 mg/g and 4.96 mg/g,respectively,which were in substantial agreement with the theoretically predicted values.