响应面法优化海参性腺酶解工艺

Optimization of Enzymatic Hydrolysis Process for Sea Cucumber Gonads by Response Surface Methodology

为有效提高海参性腺利用价值,本实验以水解度为主要指标,进行海参性腺水解蛋白酶的筛选,并考察底物浓度、酶用量、水解时间、pH值及温度等单因素对水解度的影响。在此基础上,利用响应面中心组合设计优化海参性腺水解工艺,建立水解度与底物浓度、酶用量和水解时间的二次回归模型,通过方差分析和验证实验,得出该模型能够较好地反映海参性腺水解度的变化规律。结果表明:最佳的水解工艺条件为底物浓度10.4%(m/m)、酶用量1154U/g、水解时间89min,在此条件下海参性腺水解度可达63.12%。

In order to increase the utilization value of sea cucumber gonads as a potential source of peptides,neutral protease was selected for the hydrolysis of sea cucumber gonads.One-factor-at-a-time method was used to investigate the effects of substrate concentration,enzyme dosage,hydrolysis time,pH and temperature on hydrolysis degree.Following these investigation,a quadratic regression model describing hydrolysis degree as a response to substrate concentration,enzyme dosage and hydrolysis time was set up using a Box-Behnken experimental design.As demonstrated by the results analysis of variance and validation experiments,the model could well indicate the hydrolysis degree of sea cucumber gonads.The optimal hydrolysis conditions of substrate concentration,enzyme dosage and hydrolysis time were determined to be 10.4%,1154 U/g and 89 min,respectively.Under these conditions,the hydrolysis degree of sea cucumber gonads is 63.12%.