离子色谱法直接测定食品添加剂中的甜蜜素

Direct Determination of Sodium Cyclamate in Food Additives by Ion Chromatography

建立离子色谱法直接测定食品添加剂中的甜蜜素的方法。利用甜蜜素具有较大水溶性、在水中易电离的特性,采用去离子水振荡提取样品中的甜蜜素,30mmol/L氢氧化钾溶液作为淋洗液,经阴离子交换色谱柱分离,电导检测器-离子色谱法直接测定甜蜜素。结果表明:甜蜜素含量在5～200μg/mL范围内,方法的线性关系良好(相关系数为0.9999),加标回收率为95.54%～99.24%,检出限为0.087μg/mL,方法的日内相对标准偏差小于3%,日间相对标准偏差小于2%。方法简便、稳定性好,不需对样品进行复杂的预处理,可实现对食品添加剂中甜蜜素的便捷、快速、准确测定。

A new method was developed for direct determination of sodium cyclamate in food additives by ion chromatograph coupled with electrical conductivity detector based on high water solubility and ionization characteristics of sodium cyclamate.Sodium cyclamate in samples were extracted into deionized water by shaking,separated on an anion exchange column using 30 mmol/L KOH aqueous solution as elution solvent and detected by an electrolytic conductivity detector.The method showed good linearity over the concentration range of 5-200μg/mL with a correlation coefficient of 0.9999 and a detection limit of 0.087μg/mL.The intraday and inter-day relative standard deviations（RSDs） were less than 3% and 2% respectively.The method was simple and stable without complex sample pretreatment,and allowed easy,rapid and accurate determination of sodium cyclamate in food additives.