动态连续逆流提取绞股蓝皂苷的研究

Extraction of Gypenoside from Gynostemma pentaphyllum(Thunb.) Makino by Dynamic Continuous Countercurrent Technology

本文在中试条件下,通过单因素试验和正交试验考察不同因素对绞股蓝皂苷提取得率的影响,从而探讨动态连续逆流提取绞股蓝皂苷的最佳工艺。结果表明:动态连续逆流提取绞股蓝皂苷最佳条件为:提取溶剂温度为80℃,料液比为1∶35(g/mL),提取时间为50 min。在此条件下,绞股蓝提取物平均提取得率为33.95%,皂苷得率为8.9%;动态连续逆流提取绞股蓝皂苷具有生产连续性好、皂苷提取得率高、产品纯度高等优点。

In order to determine the optimum technological parameters to extract gypenosides from Gynostemma pentaphyllum （Thunb.） Makino by using dynamic continuous countercurrent extraction,the single factor and orthogonal experiments were carried out in pilot scale test to observe the effect on extraction efficiency led by acting factors.The results showed that the optimum extraction condition were as follows：the temperature was 80 ℃,the ratio of material to solvent was 1∶35（g/mL） and the time was 50 min.In such conditions the yield of extract was 33.95% and the yield of gypenosides was 8.9%.The dynamic continuous countercurrent extraction has the advantages of better continuity,higher gypenosides yield and higher purity.