摘要
【目的】杂交育种被应用在各个物种之间。选育高品质,高产量的菌种。【方法】单孢分离技术是杂交育种行之有效的育种手段之一,近年来国内已开展这方面的研究。试验利用简单的仪器,设计一种适合鸡腿菇孢子收集和单孢分离的方法。【结果】鉴定挑选出35个单核菌株进行配对杂交,经染色镜检、拮抗试验、瓶栽试验,获得48个杂交新菌株。【结论】应用单孢分离技术对鸡腿菇进行杂交行之有效。
[ Objective ] The purpose of this project is to breed a high quality and yield fungoid. Hybridization has been taken to all of the species. [ Method] Single - Spore Isolation is one of the best way for hybridization, and many researchers have made studies in this field. With the aid of the simplest apparatus, a practical and simplest method of single - spore isolation and hybridization of Coprinus comatus was designed. [ Result] After appraisal, 35 Single -Spore mycelia were chosen for hybridization. After examination under coloration microscope, test of antagonistic lines, outgrowth generation in bottle, at last 48 new strains of filial generation were got. [ Conclusion] Using single -spore isolation to hybridize Coprinus comatus is effective.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2012年第1期190-194,共5页
Xinjiang Agricultural Sciences
基金
新疆生产建设兵团塔里木盆地生物资源保护利用重点试验室开放课题(BR0802)
新疆生产建设兵团工业科技攻关项目(2009GG38)
关键词
鸡腿菇
菌丝长势
菌丝长速
杂交
Coprinus comatus
growth potential
growth rate
hybridize