摘要
Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles （MSNP） prepared by two steps, i.e., Fe304 synthesis and silica shell growth on the surface. This magnetic nanoparticle supported ionic liquid （MNP-IL） were applied in the immobilization of penicillin G acylase （PGA）. The MSNPs and MNP-ILs were characterized by themeans of Fourier transform infrared spectroscopy （FTIR）, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, and vibrating sample magnetometer （VSM）. The results showed that the average size of magnetic Fe304 nanoparticles and MSNPs were -10 and -90 nm, respectively. The saturation magnetizations of magnetic Fe304 nanoparticles and MNP-ILs were 63.7 and 26.9 A＇m2·kg^-1, respectively. The MNP-IL was successfully applied in the immobilization of PGA. The maximum amount of loaded enzyme-was about 209 mg·g^-1 （based on carder）, and the highest enzyme activity of immobilized PGA （based on ImPGA） was 261 U·g^-1. Both the amount of loaded enzyme and the activity of ImPGA are at the same leyel of or higher than that in previous reports. After 10 consecutive operat!ons, ImPGA still mainrained 62% of its initial activity, indicating the＇good recovery property of ImPGA activity. The ionic liquid modified magnetic particles integrate the magnetic properties of Fe304 and the structure-tunable properties of ionic liquids, and have extensive potential uses in protein immobilization and magnetic bioseparation. This work may open up a novel strategy to immobilize proteins by ionic liquids.
包含 ethyoxyl 组的 Functionalized 离子的液体在二步准备的磁性的硅石 nanoparticles (MSNP ) 上被综合并且使不能调动，即，表面上的 Fe3O4 合成和硅石壳生长。这磁性的 nanoparticle 支持了离子的液体(MNP-IL ) 在青霉素 G acylase (针网阵列) 的固定被使用。MSNP 和 MNP-ILs 被 Fourier 变换的工具描绘红外线的光谱学(FTIR ) ，扫描电子显微镜学(SEM ) ，传播电子显微镜学(TEM ) ，和颤动的样品磁强计(VSM ) 。结果证明磁性的 Fe3O4 nanoparticles 和 MSNP 的平均尺寸分别地是 10 和 90 nm。磁性的 Fe3O4 nanoparticles 和 MNP-ILs 的浸透磁化是 63.7 和 26.9 A 吗？？
基金
Supported by the National Basic Research Program of China (2007CB613507)
