靶向肝素酶基因的microRNAs在胃癌中的表达

Expression of microRNAs targeting heparinase in gastric cancer

目的检测胃癌组织中肝素酶基因(heparanase,HPA)及可能靶向其表达的microRNAs(miRNAs)的表达水平。方法应用实时定量PCR检测HPA在胃癌组织中的表达变化;根据miRNAs与HPA 3'非翻译区(3'-UTR)序列的结合情况,运用生物信息学方法筛选出可能靶向HPA的miRNAs,并利用实时定量PCR检测这些miRNAs在胃癌组织中的表达水平。结果 HPA在胃癌组织中表达水平较癌旁组织和正常组织高;生物信息学分析显示,miR-18b、miR-137、miR-502和miR-299-3p可能结合于HPA的3'-UTR上,抑制其表达;实时定量PCR检测结果显示,胃癌组织miR-18b、miR-137、miR-502和miR-299-3p的表达均较癌旁组织和正常组织低。结论胃癌组织HPA高表达,而可能靶向HPA的miR-18b、miR-137、miR-502和miR-299-3p低表达,提示上述靶向HPA的miRNAs可能参与了胃癌的发生发展。

Objective To investigate the expression of heparinase（HPA） and miRNAs that may regulate HPA expression in the gastric cancer tissue.Methods The expression levels of HPA were determined by real-time PCR in the gastric cancer tissues.Based on the binding of miRNAs with the 3′ untranslated region（3′-UTR） of HPA,we screened for miRNAs targeting HPA using bioinformatics method and determined the expression levels of these miRNAs in the gastric cancer tissues using real-time PCR.Results The expression of HPA was higher in the gastric cancer tissues compared with those in the tumor-adjacent and normal tissues.Bioinformatics analysis showed that miR-18b,miR-137,miR-502,and miR-299-3p might inhibit HPA expression by binding to its 3′-UTR.Real-time PCR analysis revealed lower expression levels of miR-18b,miR-137,miR-502,and miR-299-3p in the gastric cancer tissues compared with in the adjacent and normal tissues.Conclusion HPA is highly expressed in the gastric cancer tissues;miR-18b,miR-137,miR-502 and miR-299-3p,which may target HPA,are lowly expressed,indicating miRNAs targeting HPA may participate in the carcinogenesis of gastric cancer.