二七方治疗急性放射性肠炎的疗效及可能机制

Therapeutic effects of Erqi decoction on acute radiation enteritis and the related mechanism

目的探讨二七方对急性放射性肠炎的疗效及可能的作用机制。方法 SD大鼠53只,随机分为正常组(n=8)、模型组(n=15)、白头翁汤组(n=15)及二七方组(n=15)。采用6 MV高能X线建立急性放射性肠炎大鼠模型,连续灌胃给药7 d,光学显微镜下观察及图像分析仪测定其相关的形态学指标,并测定小肠组织匀浆NO含量、SOD活性及MDA含量。结果二七方组大鼠肠组织绒毛高度[(243±9)vs(211±21)μm]、隐窝深度[(171±12)vs(142±10)μm]、黏膜[(460±21)vs(420±37)μm]及全层壁厚度[(609±19)vs(569±21)μm]均高于模型组(P<0.05),与白头翁汤组比较差异无统计学意义;二七方组大鼠肠组织匀浆NO[(0.88±0.11)vs(1.65±0.12)μmol/g]及MDA含量[(3.20±0.12)vs(4.75±0.24)nmol/mg]与模型组比较均降低(P<0.05),SOD活性升高[(212±13)vs(150±11)U/mg,P<0.05],与白头翁汤组比较差异有统计学意义(P<0.05)。结论二七方对肠黏膜结构具有保护作用,能减少肠组织NO生成、减轻炎症反应及组织过氧化损伤,抗自由基损伤可能是其治疗急性放射性肠炎的机制之一。

Objective To investigate the therapeutic effects of Erqi decoction（EQD） for treatment of acute radiation enteritis in SD rats and the related mechanism.Methods A total of 53 SD rats were randomly divided into normal group model group,EQD group and Baitouwengtang（BTWT） group,with 8 rats in the normal control group and 15 in each of the rest groups.Rats in the latter three groups were exposed to a single dose of 10 Gy 6 MV higher-energy X-rays on the abdominal region to establish the acute radiation enteritis models.Then all the rats were intragastrically administered with corresponding agents for a consecutive of 7 days.Then 10 ml ileum samples in ileocecal junction were obtained from each animal under general anesthesia.The morphologic indices were examined by light microscopy and the image analysis system.The nitric oxide（NO） levels,superoxide dismutase（SOD） activities and malondialdehyde（MDA） levels in the small intestinal homogenates were measured by spectrophotometer.Results The EQD group had significantly higher villus height（243±9 vs 211±21 μm）,deeper crypt（171±12 vs 142±10 μm）,thicker mucosa（460±21 vs 420±37 μm） and entire wall thickness（609±19 vs 569±21 μm） than the model group（P0.05）,and the above parameters were not significantly different between the EQD group and the BTWT group.EQ group had significantly lower NO level（0.88±0.11 vs 1.65±0.12 μmol/g）,higher MDA level（3.20±0.12 vs 4.75±0.24 nmol/mg） and lower SOD activity（212±13 vs 150±11 U/mg,P0.05） in the intestine compared with the model group（P0.05）.EQD group had significantly lower NO and MDA levels and higher SOD activity compared with the BTWT group（P0.05）.Conclusion It is indicated that EQD has protective effect against intestine mucosa damage in acute radiation enteritis rat model by reducing the intestinal NO generation,improving intestinal SOD activity,reducing MDA generation.Anti-free radical injury may be one of the mechanisms for treatment of acute radiation enteritis.