Vasostatin基因对胰腺癌内皮细胞迁移的影响

Inhibitory effect of vasostatin on migration of pancreatic cancer endothelial cells.

目的观察Vasostatin基因表达对胰腺癌内皮细胞迁移能力的影响。方法应用不同浓度的载有Vasostatin基因的腺病毒（Ad—vasostatin）感染胰腺癌内皮细胞，以Ad—lacZ感染及磷酸盐缓冲液（PBS）作为对照组。应用损伤修复、Transwell小室、小管形成3种不同方法观察胰腺癌内皮细胞迁移能力的变化及其与感染病毒的量效关系。结果培养48h后，PBS组与Ad—lacZ组的划痕损伤区域几乎完全恢复；而Ad-vasostatin组的划痕损伤区域无明显恢复。以MOI1、2、5感染细胞，Ad．1acZ组的迁移率分别为（84．7±2．6）％、（80．7±1．7）％和（81．3±4．0）％；Ad—vasostatin组为（77．7±2．1）％、（67．3±2．1）％和（38．8±2．1）％，Ad—vasostatin呈剂量依赖性抑制细胞迁移率，MOI：5时的细胞迁移率大幅度降低（F=180．88，P〈0．05）。以MOI1、5、10感染时，Ad-lacZ组的小管形成数分别为（118±6）、（120±6）、（82±5）个；Ad—vasostatin组为（65±4）、（21±4）、（4±1）个，Ad．vasostatin呈剂量依赖性抑制胰腺癌内皮细胞的小管形成，在MOI=10时已很难形成管状结构（F=300．85，P〈0．05）。结论Vasostatin基因能显著抑制胰腺癌内皮细胞的体外迁移能力，且呈剂量依赖性。

Objective To investigate the effect of vasostatin on the migration of pancreatic cancer endothelial cells. Methods Ad-vasostatin with different concentrations of vasostatin was used to transfect pancreatic cancer endothelial ceils. Ad-LacZ transfection and PBS was used as control. The effect of vasostatin gene mediated by adenovirus on the migration of pancreatic cancer endothelial cells was measured by wound- healing assay, transwell migration assay, and tube formation assay. Results The scratched lines in PBS group and Ad-LacZ group were almost healed 48 hours later, while the lines in Ad-vasostatin group were rarely healed. At the MOI of 1,2, 5, the migration rate of Ad-Laz group was （84.7± 2.6） %, （80.7± 1.7） % and （81.3±4.0）%, while the corresponding values were （77.7 ±2.1）%, （67.3 ±2.1）% and （38.8 ±2.1 ）% in Ad-vasostatin group. Transwell migration assay indicated that the number of migrated cells in Ad- vasostatin group was inhibited in a dose-dependant manner, at the MOI of 5, the migration became significantly decreased （F = 180.88, P 〈 0. 05）. At the MOI of 1, 5, 10, the number of tubes in Ad-LacZ group was 118 ±6, 120 ±6 and 82±5, while the corresponding values were 65 -±4, 21 ±4 and 4 ± 1 in Ad-vasostatin group. The number of tubes of pancreatic cancer endolhelial cells was inhibited by Ad-vasostatin in a dose-dependant manner, at the MOI of 10, it was difficult to form tile tubes （F =300.85, P 〈0.05）. Conclusions The vasostatin gene mediated by adenovirus has a significanl inhibitory effect on the migration of pancreatic cancer endothelial cells in vitro in a dose-dependent manner.