矿化诱导对骨髓基质细胞修复Ⅱ度根分叉病变的影响

Effects of Osteogenic Induction on Bone Marrow Mesenchymal Stromal Cells' Ability to Repair Grade Ⅱ Root Furcation Lesions

目的:研究价矿化诱导对骨髓基质细胞(bone marrow mesenchymal cells,BMSCs)体内形成牙周支持组织,治疗根分叉病变的影响。方法:2只beagle犬双侧下颌第3、4前磨牙和第1磨牙制备根分叉病变,分别植入胶原膜(空白对照组)、胶原膜-矿化诱导的BMSCs复合物(矿化诱导组)和胶原膜-非矿化诱导的BMSCs复合物(非矿化诱导组),术后12周组织学观察牙周组织再生情况。结果:新生牙槽骨面积和新生牙骨质长度的百分比,矿化诱导组(62.31±15.45和72.34±17.42)和非矿化诱导组(48.24±12.34和52.25±15.45)显著高于空白组(24.65±10.35和25.34±9.45)(P<0.05),但矿化诱导组和非矿化诱导组之间没有统计学差异。结论:BMSCs移植可以促进II度根分叉病变牙周组织再生,矿化诱导不能提高BMSCs体内修复Ⅲ度根分叉病变的能力。

Objective： To evaluate the effects of osteogenic induction on bone marrow mesenchymal stromal cells （BMSCs） ability to regenerate periodontal tissue and repair grade Ⅱ root furcation lesions. Methods： Grade Ⅱ furcation lesions were established at bilateral 3rd premolar, 4th premolar and 1st molar in two beagle dogs, and were treated by implantation of blank collagen membrane （blank control group）, collagen membrane-osteogenically in- ducted BMSCs complex （Osteogenic induction group） or collagen membrane-non-osteogenically inducted BMSCs complex （Non-osteogenical induction group）. Periodontal regeneration was evaluated histologically 12 weeks after implantion. Results： The percentage of newly formed alveolar bone and cementum was significantly higher in osteo- genic （62.31±15.45 and 72.34±17.42 respectively） and non-osteogenic induction group（48.24±12.34 and 52. 25±15.45） than in blank control group （24.65±10.35 and 25.34±9.45） （P〈0.05）, but no statistical difference was observed between osteogenic and non-osteogenic induction group. Conclusion： Implantation of BMSCs enhanced periodontal regeneration in grade Ⅲ furcation lesions, but such effects were not improved by osteogenic induction.